Penetration of nanoparticles in flax (Linum usitatissimum L.) calli and regenerants

被引:26
作者
Kokina, Inese [1 ]
Gerbreders, Vjaceslavs [2 ]
Sledevskis, Eriks [2 ]
Bulanovs, Andrejs [2 ]
机构
[1] Daugavpils Univ, Inst Systemat Biol, LV-5401 Daugavpils, Latvia
[2] Daugavpils Univ, G Liberts Innovat Microscopy Ctr, LV-5401 Daugavpils, Latvia
关键词
Biotechnology; Silver and gold nanoparticles; Calli culture; Flax; Scanning electron microscopy; SILVER NANOPARTICLES; GOLD NANOPARTICLES; PLANTS; MEMBRANES; DELIVERY; RELEASE; EXTRACT; GROWTH; CELLS; IONS;
D O I
10.1016/j.jbiotec.2013.03.011
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
We demonstrate a method for direct delivery of metal nanoparticles to flax calli and regenerant cells by vacuum deposition of metal nanolayers on powdered hormone followed by dispersal of the combined hormone-metal in medium. The penetration and location of the gold (AuNPs) and silver (AgNPs) nanoparticles in calli and in plant regenerants were confirmed by optical absorption spectroscopy and scanning electron microscopy. We detected a significant effect of the AuNPs and AgNPs on the regeneration type of flax calli. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码:127 / 132
页数:6
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