Increased PELP1 expression in rat periodontal ligament tissue in response to estrogens treatment

被引：9

作者：

Wang, Jing ^{[1
]}

Zhu, Qiang ^{[2
]}

Song, Shujun ^{[3
]}

Dong, Jun ^{[2
]}

Shi, Lixin ^{[2
]}

Tao, Ran ^{[4
]}

Ding, Yin ^{[1
]}

Hong, Baofa ^{[2
]}

机构：

[1] Forth Mil Med Univ, Coll Stomatol, Dept Orthodont, Xian 710032, Peoples R China

[2] Gen Hosp Peoples Liberat Army, Dept Urol, Beijing 100853, Peoples R China

[3] PLA, Hosp 306, Dept Pathol & Expt Med, Beijing 100101, Peoples R China

[4] Gen Hosp Peoples Liberat Army, Dept Plast Surg, Beijing 100853, Peoples R China

来源：

JOURNAL OF MOLECULAR HISTOLOGY | 2013年 / 44卷 / 03期

关键词：

Estrogen; PELP1; Ovariectomized rats; Periodontal ligament; RECEPTOR COACTIVATOR PELP1/MNAR; NONGENOMIC ACTIVITY; POTENTIAL ROLE; STEM-CELLS; ER-ALPHA; MNAR; BETA; CONTRACEPTIVES; COREGULATOR; ACTIVATION;

D O I：

10.1007/s10735-013-9490-y

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Estrogens and their receptors are important factors involved in periodontal ligament (PDL) tissue health. As a regulator of estrogen receptors (ER), the proline-, glutamic acid-, and leucine-rich protein 1 (PELP1) may play a role in alveolar bone formation and PDL homeostasis. The aim of the present study was to observe PELP1 expression in rat PDL tissue during estrogen levels manipulations. Twenty-one 8-week old normal female Sprague-Dawley rats were randomly divided into three equal groups: sham-operated controls, ovariectomized (OVX) group, and OVX given 17 beta-estradiol intraperitoneally (OVX + E-2) for 16 weeks. PELP1 expression was down-regulated in the OVX group and was up-regulated in the OVX + E-2 group. Periodontal ligament fibroblast cells (PDLFCs) were isolated from PDL tissue, and characterized by immunohistochemical staining. Estradiol treatment of PDLFCs induced PELP1 protein level compared to untreated cells. PELP1 mRNA expression in estradiol-treated cells was relatively low at the beginning of treatment and then steadily increased from hour 4. In conclusion, results indicate that PELP1 is expressed in rat PDL tissue and PDLFCs, and that its expression is up-regulated during estrogen treatment.

引用

页码：347 / 356

页数：10

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