Viral detection using a multiplex polymerase chain reaction-based assay in outpatients with upper respiratory infection

被引：7
作者：
Leekha, Surbhi [1 ]
Irish, Cole L. [2 ]
Schneider, Susan K. [2 ]
Fernholz, Emily C. [2 ]
Espy, Mark J. [2 ]
Cunningham, Scott A. [2 ]
Patel, Robin [2 ]
Juhn, Young J. [3 ]
Pritt, Bobbi [2 ]
Smith, Thomas F. [2 ]
Sampathkumar, Priya [1 ]
机构：
[1] Mayo Clin, Div Infect Dis, Rochester, MN 55905 USA
[2] Mayo Clin, Div Clin Microbiol, Rochester, MN 55905 USA
[3] Mayo Clin, Dept Pediat & Adolescent Med, Rochester, MN 55905 USA
来源：
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2013年 / 75卷 / 02期
关键词：
Multiplex PCR; Respiratory virus; Nasal swab; Throat swab; Group A streptococcus; VIRUSES; PCR; SYMPTOMS; CHILDREN; RHINOVIRUS; CULTURE; ILLNESS; SYSTEM; SWABS;
D O I：
10.1016/j.diagmicrobio.2012.10.016
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
We evaluated a commercial multiplex polymerase chain reaction (PCR) assay in a cross-sectional study among 81 adult and pediatric outpatients-40 cases with upper respiratory infection symptoms and 41 asymptomatic controls from February to April 2008. Two specimens (throat swab and nasal swab) from each participant were tested using the EraGen MultiCode-PLx Respiratory Virus Panel that detects 17 viral targets. Throat swabs were also tested for Group A Streptococcus (GAS) by PCR. Respiratory viruses were detected in 22/40 (55%) cases and in 3/41 (7%) controls (P < 0.001). GAS was detected in 10 (25%) cases; GAS and respiratory virus co-infection was found in 4 (10%). Agreement between nasal and throat swabs for viral detection was 69% in cases and 95% in controls. Of 22 cases with a detectable virus, 12 (54%) were picked up by only 1 (throat or nasal) specimen, and the detection rate was increased by combining results of nasal and throat swab testing. (C) 2013 Elsevier Inc. All rights reserved.
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页码：169 / 173
页数：5
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