Dried Urine Microsampling Coupled to Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) for the Analysis of Unconjugated Anabolic Androgenic Steroids

被引：20

作者：

Protti, Michele ^{[1
]}

Marasca, Camilla ^{[1
]}

Cirrincione, Marco ^{[1
]}

Sberna, Angelo E. ^{[2
]}

Mandrioli, Roberto ^{[3
]}

Mercolini, Laura ^{[1
]}

机构：

[1] Univ Bologna, Dept Pharm & Biotechnol FaBiT, Res Grp Pharmaco Toxicol Anal, PTA Lab,Alma Mater Studiorum, I-40126 Bologna, Italy

[2] Enna Local Hlth Unit, Sport Med, I-94100 Enna, Italy

[3] Univ Bologna, Dept Life Qual Studies, Alma Mater Studiorum, I-47921 Rimini, Italy

来源：

MOLECULES | 2020年 / 25卷 / 14期

关键词：

anabolic androgenic steroids; anti-doping; dried urine spots (DUS); LC-MS; MS; microsampling; neuroprotection; volumetric absorptive microsampling (VAMS); CONFIRMATION; ESI/MS/MS; PLASMA;

D O I：

10.3390/molecules25143210

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Testing and monitoring anabolic androgenic steroids in biological fluids is a key activity in anti-doping practices. In this study, a novel approach is proposed, based on dried urine microsampling through two different workflows: dried urine spots (DUS) and volumetric absorptive microsampling (VAMS). Both techniques can overcome some common drawbacks of urine sampling, such as analyte instability and storage and transportation problems. Using an original, validated liquid chromatography-tandem mass spectrometry (LC-MS/MS) method, exogenous and endogenous unconjugated steroids were analysed. Despite the limitations of microsampling volume, good sensitivity was obtained (limit of quantitation <= 1.5 ng/mL for all analytes), with satisfactory precision (relative standard deviation <7.6%) and absolute recovery (>70.3%). Both microsampling platforms provide reliable results, in good agreement with those obtained from urine.

引用

页数：18

共 31 条

[1] Simple liquid chromatography method for the rapid simultaneous determination of prednisolone and cortisol in plasma and urine using hydrophilic lipophilic balanced solid phase extraction cartridges [J].