Crystal ball: Fluorescence in situ hybridization in the age of super-resolution microscopy

被引：10

作者：

Moraru, Cristina ^{[1
]}

Amann, Rudolf ^{[1
]}

机构：

[1] Max Planck Inst Marine Microbiol, D-28359 Bremen, Germany

来源：

SYSTEMATIC AND APPLIED MICROBIOLOGY | 2012年 / 35卷 / 08期

关键词：

Fluorescence in situ hybridization; Super-resolution microscopy; rRNA FISH; Environmental microbiology; IDENTIFICATION; RIBOSOMES; PROBES; CELLS;

D O I：

10.1016/j.syapm.2012.10.001

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Super-resolution microscopy encompasses a suite of cutting edge microscopy methods able to surpass the resolution limits of light microscopy. The recent commercial availability of super-resolution microscopy is advancing many fields of biology. In this crystal ball forward look, we briefly examine the perspectives of combining super-resolution microscopy and fluorescence in situ hybridization (FISH). We strongly believe, based on first evidence presented here, that using super-resolution microscopy in environmental microbiology has the potential to reshape the way we analyze the results obtained with FISH, by improving both the localization and quantification of target molecules. (c) 2012 Elsevier GmbH. All rights reserved.

引用

页码：549 / 552

页数：4

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