Recombinant rabies virus expressing IL-21 enhances immunogenicity through activation of T follicular helper cells and germinal centre B cells

被引：16

作者：

Zhang, Yajing ^{[1
,2
]}

Zhou, Ming ^{[1
,2
]}

Wang, Zhao ^{[1
,2
]}

Yang, Jie ^{[1
,2
]}

Li, Mingming ^{[1
,2
]}

Wang, Kunlun ^{[1
,2
]}

Cui, Min ^{[1
,2
]}

Chen, Huanchun ^{[1
,2
]}

Fu, Zhen F. ^{[1
,2
,3
]}

Zhao, Ling ^{[1
,2
]}

机构：

[1] Huazhong Agr Univ, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China

[2] Huazhong Agr Univ, Coll Vet Med, Wuhan 430070, Peoples R China

[3] Univ Georgia, Dept Pathol, Athens, GA 30602 USA

来源：

JOURNAL OF GENERAL VIROLOGY | 2016年 / 97卷

基金：

中国国家自然科学基金;

关键词：

DENDRITIC CELLS; GM-CSF; INTERLEUKIN-21; GENERATION; VACCINE; PATHOGENICITY; CYTOKINES; SEQUENCE; IMMUNITY;

D O I：

10.1099/jgv.0.000646

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Previous studies have demonstrated that the lack of interleukin-21 (IL-21) signalling could affect specific antibody induction after rabies vaccination. Here, to further investigate the overexpression of IL-21 on the immunogenicity of rabies virus (RABV), a recombinant RABV expressing murine IL-21, designated LBNSE-IL21, was constructed and evaluated in a mouse model. It was found that in mice immunized with LBNSE-IL21, there was a substantial increase in the number of T follicular helper cells and germinal centre B cells but no enhancement of dendritic cell activation. Furthermore, significantly higher rabies virus-neutralizing antibody (VNA) titres were produced in mice immunized with LBNSE-IL21 than in mice immunized with the parent virus LBNSE in the first six weeks, resulting in higher protection. Together, these results suggest that LBNSE-IL21 can induce a rapid and robust VNA titre, and it has the potential to be developed as a promising rabies vaccine.

引用

页码：3154 / 3160

页数：7

共 29 条

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