Carvacrol inhibits proliferation and induces apoptosis in human colon cancer cells

被引:112
作者
Fan, Kai [1 ]
Li, Xiaolei [2 ]
Cao, Yonggang [3 ]
Qi, Hanping [3 ]
Li, Lei [4 ]
Zhang, Qianhui [3 ]
Sun, Hongli [3 ]
机构
[1] Harbin Med Univ Daqing, Dept Pathophysiol, Daqing 8153081, Heilongjiang, Peoples R China
[2] Harbin Med Univ Daqing, Dept Pathol, Daqing 8153081, Heilongjiang, Peoples R China
[3] Harbin Med Univ Daqing, Dept Pharmacol, Daqing 8153081, Heilongjiang, Peoples R China
[4] Harbin Med Univ Daqing, Clin Coll 5, Dept Surg, Daqing 8153081, Heilongjiang, Peoples R China
关键词
apoptosis; carvacrol; colon cancer; proliferation; CYCLE ARREST; PI3K/AKT; PATHWAY; EXPRESSION; MIGRATION; INVASION; BEARING; MATRIX;
D O I
10.1097/CAD.0000000000000263
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Colon cancer is one of the most common malignancies worldwide and has a high mortality rate. Carvacrol is a major component of oregano and thyme essential oils and shows antitumor properties. Here, we investigated the effects of carvacrol on the proliferation and apoptosis of two human colon cancer cell lines, HCT116 and LoVo, and studied the molecular mechanisms of its antitumor properties. We found that carvacrol inhibited the proliferation and migration of the two colon cancer cell lines in a concentration-dependent manner. Cell invasion was suppressed after carvacrol treatment by decreasing the expression of matrix metalloprotease-2 (MMP-2) and MMP-9. Carvacrol treatment also caused cell cycle arrest in the G2/M phase and decreased cyclin B1 expression. Finally, carvacrol induced cell apoptosis in a dose-dependent manner. At the molecular level, carvacrol downregulated the expression of Bcl-2 and induced the phosphorylation of the extracellular-regulated protein kinase and protein kinase B (p-Akt). In parallel, carvacrol upregulated the expression of Bax and c-Jun N-terminal kinase. These results indicate that carvacrol might induce apoptosis in colon cancer cells through the mitochondrial apoptotic pathway and the MAPK and PI3K/Akt signaling pathways. Together, our results suggest that carvacrol may have therapeutic potential for the prevention and treatment of colon cancer. Copyright (C) 2015 Wolters Kluwer Health, Inc. All rights reserved.
引用
收藏
页码:813 / 823
页数:11
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