A preclinical microbeam facility with a conventional x-ray tube

被引:21
作者
Bartzsch, Stefan [1 ]
Cummings, Craig [1 ]
Eismann, Stephan [2 ]
Oelfke, Uwe [1 ]
机构
[1] Inst Canc Res, 15 Cotswold Rd, Belmont Sutton SM2 5NG, Surrey, England
[2] Heidelberg Univ, Dept Phys & Astron, Grabengasse 1, D-69117 Heidelberg, Germany
关键词
microbeam radiation therapy; compact microbeam sources; dosimetry; collimators; cellular response; RADIATION-THERAPY; SYNCHROTRON-WIGGLER; IN-VIVO; RADIOTHERAPY; IRRADIATION; BYSTANDER; DESIGN; SYSTEM; CELL; MRT;
D O I
10.1118/1.4966032
中图分类号
R8 [特种医学]; R445 [影像诊断学];
学科分类号
1002 ; 100207 ; 1009 ;
摘要
Purpose: Microbeam radiation therapy is an innovative treatment approach in radiation therapy that uses arrays of a few tens of micrometer wide and a few hundreds of micrometer spaced planar x-ray beams as treatment fields. In preclinical studies these fields efficiently eradicated tumors while normal tissue could effectively be spared. However, development and clinical application of microbeam radiation therapy is impeded by a lack of suitable small scale sources. Until now, only large synchrotrons provide appropriate beam properties for the production of microbeams. Methods: In this work, a conventional x-ray tube with a small focal spot and a specially designed collimator are used to produce microbeams for preclinical research. The applicability of the developed source is demonstrated in a pilot in vitro experiment. The properties of the produced radiation field are characterized by radiochromic film dosimetry. Results: 50 mu m wide and 400 mu m spaced microbeams were produced in a 20x20 mm(2) sized microbeam field. The peak to valley dose ratio ranged from 15.5 to 30, which is comparable to values obtained at synchrotrons. A dose rate of up to 300 mGy/s was achieved in the microbeam peaks. Analysis of DNA double strand repair and cell cycle distribution after in vitro exposures of pancreatic cancer cells (Panc1) at the x-ray tube and the European Synchrotron leads to similar results. In particular, a reduced G2 cell cycle arrest is observed in cells in the microbeam peak region. Conclusions: At its current stage, the source is restricted to in vitro applications. However, moderate modifications of the setup may soon allow in vivo research in mice and rats. (C) 2016 Author(s).
引用
收藏
页码:6301 / 6308
页数:8
相关论文
共 31 条
[21]   PATHOLOGIC AND BEHAVIORAL CHANGES IN MICE AFTER DEUTERON IRRADIATION OF CENTRAL NERVOUS SYSTEM [J].
ORDY, JM ;
SAMORAJSKI, T ;
CURTIS, HJ ;
BARNES, HW ;
WOLIN, L ;
ZEMAN, W .
RADIATION RESEARCH, 1963, 18 (01) :31-&
[22]   Dosimetry protocol for the preclinical trials in white-beam minibeam radiation therapy [J].
Prezado, Y. ;
Martinez-Rovira, I. ;
Thengumpallil, S. ;
Deman, P. .
MEDICAL PHYSICS, 2011, 38 (09) :5012-5020
[23]  
Prise Kevin M., 2006, Dose-Response, V4, P263, DOI 10.2203/dose-response.06-113.Prise
[24]   The radiotherapy clinical trials projects at the ESRF: Technical aspects [J].
Renier, M. ;
Brochard, Th. ;
Nemoz, C. ;
Requardt, H. ;
Brauer, E. ;
Esteve, F. ;
Balosso, J. ;
Suortti, P. ;
Baruchel, J. ;
Elleaume, H. ;
Berruyer, G. ;
Berkvens, P. ;
Bravin, A. .
EUROPEAN JOURNAL OF RADIOLOGY, 2008, 68 (03) :S147-S150
[25]   MICROBEAM RADIATION-INDUCED TISSUE DAMAGE DEPENDS ON THE STAGE OF VASCULAR MATURATION [J].
Sabatasso, Sara ;
Laissue, Jean Albert ;
Hlushchuk, Ruslan ;
Graber, Werner ;
Bravin, Alberto ;
Braeuer-Krisch, Elke ;
Corde, Stephanie ;
Blattmann, Hans ;
Gruber, Guenther ;
Djonov, Valentin .
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS, 2011, 80 (05) :1522-1532
[26]   Monte Carlo simulation of a compact microbeam radiotherapy system based on carbon nanotube field emission technology [J].
Schreiber, Eric C. ;
Chang, Sha X. .
MEDICAL PHYSICS, 2012, 39 (08) :4669-4678
[27]   In vivo two-photon microscopy study of short-term effects of microbeam irradiation on normal mouse brain microvasculature [J].
Serduc, R ;
Vérant, P ;
Vial, JC ;
Farion, R ;
Rocas, L ;
Rémy, C ;
Fadlallah, T ;
Brauer, E ;
Bravin, A ;
Laissue, J ;
Blattmann, H ;
Van der Sanden, B .
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS, 2006, 64 (05) :1519-1527
[28]   Characterization and quantification of cerebral edema induced by synchrotron x-ray microbeam radiation therapy [J].
Serduc, Raphael ;
de Looij, Yohan Van ;
Francony, Gilles ;
Verdonck, Olivier ;
Van der Sanden, Boudewijn ;
Laissue, Jean ;
Farion, Regine ;
Braeuer-Krisch, Elke ;
Siegbahn, Erik Albert ;
Bravin, Alberto ;
Prezado, Yolanda ;
Segebarth, Christoph ;
Remy, Chantal ;
Lahrech, Hana .
PHYSICS IN MEDICINE AND BIOLOGY, 2008, 53 (05) :1153-1166
[29]   MICROBEAM RADIATION-THERAPY [J].
SLATKIN, DN ;
SPANNE, P ;
DILMANIAN, FA ;
SANDBORG, M .
MEDICAL PHYSICS, 1992, 19 (06) :1395-1400
[30]   SUBACUTE NEUROPATHOLOGICAL EFFECTS OF MICROPLANAR BEAMS OF X-RAYS FROM A SYNCHROTRON WIGGLER [J].
SLATKIN, DN ;
SPANNE, P ;
DILMANIAN, FA ;
GEBBERS, JO ;
LAISSUE, JA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (19) :8783-8787