Antioxidant and hemolytic activities, and effects in rat cutaneous wound healing of a novel polysaccharide from fenugreek (Trigonella foenum-graecum) seeds

被引：56

作者：

Ktari, Naourez ^{[1
]}

Trabelsi, Imen ^{[2
]}

Bardaa, Sana ^{[3
]}

Triki, Mehdi ^{[2
]}

Bkhairia, Intidhar ^{[1
,2
]}

Ben Slama-Ben Salem, Rabab ^{[1
]}

Nasri, Moncef ^{[1
]}

Ben Salah, Riadh ^{[2
]}

机构：

[1] Univ Sfax, Natl Sch Engn Sfax, Lab Enzyme Engn & Microbiol, POB 1173-3038, Sfax, Tunisia

[2] Ctr Biotechnol Sfax, Lab Microorganisms & Biomol LMB, Rd Sidi Mansour Km 6,POB 1177, Sfax 3018, Tunisia

[3] Univ Sfax, Fac Med Sfax, Pharmacol Lab, Ave Majida Boulila, Sfax 3029, Tunisia

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2017年 / 95卷

关键词：

Polysaccharide; Fenugreek seeds; Antioxidant activities; Hemolytic activity; Wound healing; WATER-SOLUBLE POLYSACCHARIDES; CHEMICAL-COMPOSITION; ASSISTED EXTRACTION; IN-VITRO; OPTIMIZATION; SALICYLATE;

D O I：

10.1016/j.ijbiomac.2016.11.091

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of this work was to evaluate the antioxidant and hemolytic activities as well as the in vivo wound healing performance of a novel polysaccharide (FWEP) extracted from fenugreek (Trigonella foenumgraecum) seeds. The antioxidant activity was evaluated in vivo and in vitro using various assays. Results showed that FWEP exhibited strong antioxidant activities but no hemolytic activity was observed towards bovine erythrocytes. The application of FWEP hydrogel on the wound site in a rat model enhanced significantly wound healing activity and accelerated the wound closure after 14 days of wound induction. Histological examination also demonstrated fully re-epithelialized wound with a complete epidermal regeneration. Altogether, these evidences demonstrated that FWEP had strong wound healing potential presumably achieved through its antioxidant activities. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：625 / 634

页数：10

共 61 条

[1] Abdel-Azim N. S., 2011, Research Journal of Medicinal Plant, V5, P136, DOI 10.3923/rjmp.2011.136.144
[2] Purification, characterization and antioxidant activity of polysaccharides extracted from the fibrous pulp of Mangifera pajang fruits
Al-Sheraji, Sadeq Hassan
Ismail, Amin
Manap, Mohd Yazid
Mustafa, Shuhaimi
Yusof, Rokiah Mohd
Hassan, Fouad Abdulrahman
[J]. LWT-FOOD SCIENCE AND TECHNOLOGY, 2012, 48 (02) : 291 - 296
[3] [Anonymous], 2006, HEMOSTASIS THROMBOSI
[4] AOAC (Association of Official Analytical Chemists), 2000, METH AN GAITH
[5] Reactive oxygen species and their detoxification in healing skin wounds
auf dem Keller, Ulrich
Kuemin, Angelika
Braun, Susanne
Werner, Sabine
[J]. JOURNAL OF INVESTIGATIVE DERMATOLOGY SYMPOSIUM PROCEEDINGS, 2006, 11 (01) : 106 - 111
[6] Promotion of dermal wound healing by polysaccharides isolated from Phellinus gilvus in rats
Bae, JS
Jang, KH
Park, SC
Jin, HK
[J]. JOURNAL OF VETERINARY MEDICAL SCIENCE, 2005, 67 (01) : 111 - 114
[7] Structural, functional, and antioxidant properties of water-soluble polysaccharides from potatoes peels
Ben Jeddou, Khawla
Chaari, Fatma
Maktouf, Sameh
Nouri-Ellouz, Oumema
Helbert, Claire Boisset
Ghorbel, Raoudha Ellouz
[J]. FOOD CHEMISTRY, 2016, 205 : 97 - 105
[8] Antioxidants from a heated histidine-glucose model system. I: Investigation of the antioxidant role of histidine and isolation of antioxidants by high-performance liquid chromatography
Bersuder, P
Hole, M
Smith, G
[J]. JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1998, 75 (02) : 181 - 187
[9] Buege J.A., 1972, METHOD ENZYMOL, V52, P302
[10] Extraction, characterization and antioxidant activity of water-soluble polysaccharides from Tuber huidongense
Chen, Guangjing
Zhang, Shiqi
Ran, Chunxia
Wang, Lisha
Kan, Jianquan
[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2016, 91 : 431 - 442

← 1 2 3 4 5 6 7 →