Determination and Pharmacokinetics of Di-(2-ethylhexyl) Phthalate in Rats by Ultra Performance Liquid Chromatography with Tandem Mass Spectrometry

被引:17
作者
Chang-Liao, Wan-Ling [1 ]
Hou, Mei-Ling [1 ]
Chang, Li-Wen [1 ]
Lee, Chia-Jung [1 ]
Tsai, Yin-Meng [1 ]
Lin, Lie-Chwen [1 ,2 ]
Tsai, Tung-Hu [1 ,3 ,4 ]
机构
[1] Natl Yang Ming Univ, Sch Med, Inst Tradit Med, Taipei 112, Taiwan
[2] Natl Res Inst Chinese Med, Taipei 11221, Taiwan
[3] China Med Univ, Grad Inst Acupuncture Sci, Taichung 404, Taiwan
[4] Taipei City Hosp, Dept Educ & Res, Taipei 103, Taiwan
关键词
di-(2-ethylhexyl) phthalate (DEHP); fecal excretion; pharmacokinetics; phthalates; plasticizer; DI(2-ETHYLHEXYL) PHTHALATE; POLYVINYL-CHLORIDE; WATER SAMPLES; HUMAN URINE; UPLC-MS/MS; DEHP; METABOLISM; EXPOSURE; TOXICITY; PLASTICIZER;
D O I
10.3390/molecules180911452
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Di-(2-ethylhexyl) phthalate (DEHP) is used to increase the flexibility of plastics for industrial products. However, the illegal use of the plasticizer DEHP in food and drinks has been reported in Taiwan in 2011. In order to assess the exact extent of the absorption of DEHP via the oral route, the aim of this study is to develop a reliable and validated ultra performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) method to evaluate the oral bioavailability of DEHP in rats. The optimal chromatographic separation of DEHP and butyl benzyl phthalate (BBP; used as internal standard) were achieved on a C-18 column. The mobile phase was consisted of 5 mM ammonium acetate-methanol (11:89, v/v) with a flow rate of 0.25 mL/min. The monitoring ion transitions were m/z 391.4 -> 149.0 for DEHP and m/z 313.3 -> 149.0 for BBP. The mean matrix effects of DEHP at low, medium and high concentrations were 94.5 +/- 5.7% and 100.1 +/- 2.3% in plasma and feces homogenate samples, respectively. In conclusion, the validated UPLC-MS/MS method is suitable for analyzing the rat plasma sample of DEHP and the oral bioavailability of DEHP was about 7% in rats.
引用
收藏
页码:11452 / 11466
页数:15
相关论文
共 46 条
[11]   A cancer risk assessment of di(2-ethylhexyl)phthalate: Application of the new US EPA risk assessment guidelines [J].
Doull, J ;
Cattley, R ;
Elcombe, C ;
Lake, BG ;
Swenberg, J ;
Wilkinson, C ;
Williams, G ;
van Gemert, M .
REGULATORY TOXICOLOGY AND PHARMACOLOGY, 1999, 29 (03) :327-357
[12]   Prediction of rodent nongenotoxic carcinogenesis: Evaluation of biochemical and tissue changes in rodents following exposure to nine nongenotoxic NTP carcinogens [J].
Elcombe, CR ;
Odum, J ;
Foster, JR ;
Stone, S ;
Hasmall, S ;
Soames, AR ;
Kimber, I ;
Ashby, J .
ENVIRONMENTAL HEALTH PERSPECTIVES, 2002, 110 (04) :363-375
[13]   Stability, compatibility and plasticizer extraction of quinine injection added to infusion solutions and stored in polyvinyl chloride (PVC) containers [J].
Faouzi, MA ;
Khalfi, F ;
Dine, T ;
Luyckx, M ;
Brunet, C ;
Gressier, B ;
Goudaliez, F ;
Cazin, M ;
Kablan, J ;
Belabed, A ;
Cazin, JC .
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1999, 21 (05) :923-930
[14]   Experimental study on infusion devices containing polyvinyl chloride: To what extent are they di(2-ethylhexyl)phthalate-free? [J].
Genay, Stephanie ;
Luciani, Chiara ;
Decaudin, Bertrand ;
Kambia, Nicolas ;
Dine, Thierry ;
Azaroual, Nathalie ;
Di Martino, Piera ;
Barthelemy, Christine ;
Odou, Pascal .
INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2011, 412 (1-2) :47-51
[16]   Investigation of the release behavior of diethylhexyl phthalate from the polyvinyl-chloride tubing for intravenous administration [J].
Hanawa, T ;
Muramatsu, E ;
Asakawa, K ;
Suzuki, M ;
Tanaka, M ;
Kawano, K ;
Seki, T ;
Juni, K ;
Nakajima, S .
INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2000, 210 (1-2) :109-115
[17]  
HILLMAN LS, 1975, NEW ENGL J MED, V292, P381, DOI 10.1056/NEJM197502202920801
[18]   Pharmacokinetics of di-isononyl phthalate in freely moving rats by UPLC-MS/MS [J].
Hou, Mei-Ling ;
Chang, Li-Wen ;
Chiang, Chang-Jung ;
Tsuang, Yang-Hwei ;
Lin, Chi-Hung ;
Tsai, Tung-Hu .
INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2013, 450 (1-2) :36-43
[19]   Hepatocarcinogenic potential of di(2-ethylhexyl)phthalate in rodents and its implications on human risk [J].
Huber, WW ;
GraslKraupp, B ;
SchulteHermann, R .
CRITICAL REVIEWS IN TOXICOLOGY, 1996, 26 (04) :365-481
[20]   Column-switching high-performance liquid chromatography electrospray mass spectrometry coupled with on-line of extraction for the determination of mono- and di-(2-ethylhexyl) phthalate in blood samples [J].
Inoue, K ;
Kawaguchi, M ;
Okada, F ;
Yoshimura, Y ;
Nakazawa, H .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2003, 375 (04) :527-533