HIGH-THROUGHPUT, HIGH-FREQUENCY 3-D ULTRASOUND FOR IN UTERO ANALYSIS OF EMBRYONIC MOUSE BRAIN DEVELOPMENT

被引：29

作者：

Aristizabal, Orlando ^{[1
,2
,3
]}

Mamou, Jonathan ^{[3
]}

Ketterling, Jeffrey A. ^{[3
]}

Turnbull, Daniel H. ^{[1
,2
]}

机构：

[1] NYU, Sch Med, Skirball Inst Biomol Med, Kimmel Ctr Biol & Med, New York, NY 10016 USA

[2] NYU, Dept Radiol, Sch Med, New York, NY 10016 USA

[3] Riverside Res, Lizzi Ctr Biomed Engn, New York, NY 10038 USA

来源：

ULTRASOUND IN MEDICINE AND BIOLOGY | 2013年 / 39卷 / 12期

基金：

美国国家卫生研究院;

关键词：

Annular array; Central nervous system; Synthetic focusing; Coded excitation; Respiratory gating; Mouse embryo; ANNULAR ARMY TRANSDUCER; CENTRAL-NERVOUS-SYSTEM; INT-1; PROTOONCOGENE; MICRO-ULTRASOUND; LINEAR-ARRAY; VIVO; MRI; FABRICATION; BIOMICROSCOPY; BACKSCATTER;

D O I：

10.1016/j.ultrasmedbio.2013.06.015

中图分类号：

O42 [声学];

学科分类号：

070206 ; 082403 ;

摘要：

With the emergence of the mouse as the predominant model system for studying mammalian brain development, in utero imaging methods are urgently required to analyze the dynamics of brain growth and patterning in mouse embryos. To address this need, we combined synthetic focusing with a high-frequency (38-MHz) annular-array ultrasound imaging system for extended depth-of-field, coded excitation for improved penetration and respiratory-gated transmit/receive. This combination allowed non-invasive in utero acquisition of motion-free 3-D data from individual embryos in approximately 2 min, and data from four or more embryos in a pregnant mouse in less than 30 min. Data were acquired from 148 embryos spanning 5 d of early to mid-gestational stages of brain development. The results indicated that brain anatomy and cerebral vasculature can be imaged with this system and that quantitative analyses of segmented cerebral ventricles can be used to characterize volumetric changes associated with mouse brain development. (E-mail: Jketterling@RiversideResearch.org and Daniel. Turnbull@med.nyu.edu) (C) 2013 World Federation for Ultrasound in Medicine & Biology.

引用

页码：2321 / 2332

页数：12

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