A plasmid-cured Chlamydia muridarum strain displays altered plaque morphology and reduced infectivity in cell culture

被引：74

作者：

O'Connell, Catherine M. ^{[1
]}

Nicks, Kristy M. ^{[1
]}

机构：

[1] UAMS, Dept Microbiol & Immunol, Little Rock, AR 72205 USA

来源：

MICROBIOLOGY-SGM | 2006年 / 152卷

关键词：

D O I：

10.1099/mic.0.28658-0

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A highly conserved cryptic plasmid is present in Chlamydia trachomatis yet naturally occurring plasmid-deficient isolates are very rare. This paper describes the isolation and characterization of a plasmid-deficient strain of C. muridarum, using novobiocin as a curing agent. Plasmid-deficient derivatives of C. muridarum strain Nigg were generated at high efficiencies (4-30%). Phenotypic characterization revealed that the cured derivative was unable to accumulate glycogen within intracytoplasmic inclusions. In addition, this strain formed small plaques at a reduced efficiency compared to the wild-type parent.

引用

页码：1601 / 1607

页数：7

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