Genetic Architecture of Resistance to Alternaria brassicae in Arabidopsis thaliana: QTL Mapping Reveals Two Major Resistance-Conferring Loci

被引:28
作者
Rajarammohan, Sivasubramanian [1 ]
Kumar, Amarendra [1 ,3 ]
Gupta, Vibha [2 ]
Pental, Deepak [1 ,2 ]
Pradhan, Akshay K. [1 ,2 ]
Kaur, Jagreet [1 ]
机构
[1] Univ Delhi, Dept Genet, South Campus, New Delhi, India
[2] Univ Delhi, Ctr Genet Manipulat Crop Plants, South Campus, New Delhi, India
[3] Bihar Agr Univ, Dept Plant Pathol, Bhagalpur, India
来源
FRONTIERS IN PLANT SCIENCE | 2017年 / 8卷
关键词
Arabidopsis; Alternaria brassicae; disease resistance; necrotroph; QTL mapping; defense response; DOWNY MILDEW RESISTANCE; DISEASE RESISTANCE; INFECTION; SUSCEPTIBILITY; IDENTIFICATION; DNA;
D O I
10.3389/fpls.2017.00260
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Alternaria brassicae, a necrotrophic fungal pathogen, causes Alternaria blight, one of the most important diseases of oleiferous Brassica crops. The current study utilized Arabidopsis as a model to decipher the genetic architecture of defense against A. brassicae. Significant phenotypic variation that was largely genetically determined was observed among Arabidopsis accessions in response to pathogen challenge. Three biparental mapping populations were developed from three resistant accessions viz. CIBC-5, Ei-2, and Cvi-0 and two susceptible accessions - Gre-0 and Zdr-1 (commonly crossed to CIBC-5 and Ei-2). A total of six quantitative trait locus (QTLs) governing resistance to A. brassicae were identified, five of which were population-specific while one QTL was common between all the three mapping populations. Interestingly, the common QTL had varying phenotypic contributions in different populations, which can be attributed to the genetic background of the parental accessions. The presence of both common and population-specific QTLs indicate that resistance to A. brassicae is quantitative, and that different genes may mediate resistance to the pathogen in different accessions. Two of the QTLs had moderate-to-large effects, one of which explained nearly 50% of the variation. The large effect QTLs may therefore contain genes that could play a significant role in conferring resistance even in heterologous hosts.
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页码:1 / 9
页数:9
相关论文
共 32 条
[1]  
[Anonymous], NEWS LETT
[2]  
[Anonymous], ALERNARIA DIS CRUCIF
[3]   Genome-wide association mapping in Arabidopsis identifies previously known flowering time and pathogen resistance genes [J].
Aranzana, MJ ;
Kim, S ;
Zhao, KY ;
Bakker, E ;
Horton, M ;
Jakob, K ;
Lister, C ;
Molitor, J ;
Shindo, C ;
Tang, CL ;
Toomajian, C ;
Traw, B ;
Zheng, HG ;
Bergelson, J ;
Dean, C ;
Marjoram, P ;
Nordborg, M .
PLOS GENETICS, 2005, 1 (05) :531-539
[4]  
CHURCHILL GA, 1994, GENETICS, V138, P963
[5]   Identification of Botrytis cinerea susceptibility loci in Arabidopsis thaliana [J].
Denby, KJ ;
Kumar, P ;
Kliebenstein, DJ .
PLANT JOURNAL, 2004, 38 (03) :473-486
[6]   Genetic characterization of RRS1, a recessive locus in Arabidopsis thaliana that confers resistance to the bacterial soilborne pathogen Ralstonia solanacearum [J].
Deslandes, L ;
Pileur, F ;
Liaubet, L ;
Camut, S ;
Can, C ;
Williams, K ;
Holub, E ;
Beynon, J ;
Arlat, M ;
Marco, Y .
MOLECULAR PLANT-MICROBE INTERACTIONS, 1998, 11 (07) :659-667
[7]  
Doerge RW, 1996, GENETICS, V142, P285
[8]   A SIMPLE AND RAPID METHOD FOR THE PREPARATION OF PLANT GENOMIC DNA FOR PCR ANALYSIS [J].
EDWARDS, K ;
JOHNSTONE, C ;
THOMPSON, C .
NUCLEIC ACIDS RESEARCH, 1991, 19 (06) :1349-1349
[10]  
Grover A, 2003, CURR SCI INDIA, V84, P310