Development of a nested PCR and its internal control for the detection of African swine fever virus (ASFV) in Ornithodoros erraticus

被引：62

作者：

Basto, AP

Portugal, RS

Nix, RJ

Cartaxeiro, C

Boinas, F

Dixon, LK

Leitao, A

Martins, C

机构：

[1] CIISA, CVZ, Inst Invest Cient Trop, P-1300477 Lisbon, Portugal

[2] CIISA, Fac Med Vet, Lab Coencas Infecciosas, P-1300477 Lisbon, Portugal

[3] Pirbright Lab, Inst Anim Hlth, Pirbright, England

来源：

ARCHIVES OF VIROLOGY | 2006年 / 151卷 / 04期

基金：

英国生物技术与生命科学研究理事会;

关键词：

D O I：

10.1007/s00705-005-0654-2

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A nested PCR assay, with an internal control, was developed to detect African swine fever virus (ASFV) DNA in Ornithodoros erraticus. The assay revealed a better analytical sensitivity than virus isolation and the OIE PCR protocol. All ticks collected from the field, which were positive by virus isolation, were also positive by PCR. Viral DNA was detected in a further 19 out of 60 ticks from which no virus was isolated. Our results show that this assay is reliable and can easily be used to screen large tick populations collected in the field for the presence of ASFV.

引用

页码：819 / 826

页数：8

共 20 条

[1] A highly sensitive and specific gel-based multiplex RT-PCR assay for the simultaneous and differential diagnosis of African swine fever and Classical swine fever in clinical samples [J].