Tubulin isoforms identified in the brain by MALDI in-source decay

被引：8

作者：

Ait-Belkacem, Rima ^{[1
]}

Calligaris, David ^{[2
]}

Sellami, Lyna ^{[1
]}

Villard, Claude ^{[1
]}

Granjeaud, Samuel ^{[3
]}

Schembri, Therese ^{[1
]}

Berenguer, Caroline ^{[1
]}

Ouafik, L'Houcine ^{[1
]}

Figarella-Branger, Dominique ^{[1
]}

Chinot, Olivier ^{[1
]}

Lafitte, Daniel ^{[1
]}

机构：

[1] Aix Marseille Univ, INSERM, UMR911 CRO2, F-13385 Marseille, France

[2] Univ Liege, GIGA Res, Mass Spectrometry Lab, B-4000 Liege, Belgium

[3] Aix Marseille Univ, INSERM, U1068, CRCM, F-13009 Marseille, France

来源：

JOURNAL OF PROTEOMICS | 2013年 / 79卷

关键词：

Biomarker discovery; In-source decay; Tubulin; Tubulin isotypes; III BETA-TUBULIN; FRAGMENTATION; PEPTIDES; MATRIX; ALPHA; LOCALIZATION; EXPRESSION; PROTEOMICS; BETA(III); PROTEINS;

D O I：

10.1016/j.jprot.2012.11.026

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Identification of biomarkers is a major issue for enhancement of chemotherapies. The molecular characterization of tissues necessitates the identification of thousands of biomolecules each participating in physiopathological processes. MALDI in-source decay (ISD) fragmentation has already been proven to be effective for protein characterization. However, the difficulty to identify proteins from complex mixtures such as tissue sections can limit the applications of this technique. In this study, we evidenced that tubulin has an unusual fragmentation pathway in the MALDI source. This striking property allowed the detecting of several mouse brain tubulin isotypes simultaneously by simply using laser fragmentation. Tubulin isoforms are consistent markers of a bad prognosis of solid tumors and could be the target of targeted chemotherapies. Such a direct molecular printout of tubulin in tissues is a milestone that should be useful either at preclinical or clinical stage. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：172 / 179

页数：8

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