Selective Catecholamine Recognition with NeuroSensor 521: A Fluorescent Sensor for the Visualization of Norepinephrine in Fixed and Live Cells

被引：66
作者：
Hettie, Kenneth S. [1 ]
Liu, Xin [2 ]
Gillis, Kevin D. [2 ,3 ,4 ]
Glass, Timothy E. [1 ]
机构：
[1] Univ Missouri, Dept Chem, Columbia, MO 65211 USA
[2] Univ Missouri, Dalton Cardiovasc Res Ctr, Columbia, MO 65211 USA
[3] Univ Missouri, Columbia, MO 65211 USA
[4] Univ Missouri, Dept Med Pharmacol & Physiol, Columbia, MO 65211 USA
来源：
ACS CHEMICAL NEUROSCIENCE | 2013年 / 4卷 / 06期
基金：
美国国家科学基金会;
关键词：
Fluorescent sensor; catecholamine; norepinephrine; cell imaging; chromaffin; CHROMAFFIN CELLS; DOPAMINE; EXOCYTOSIS; NEUROTRANSMITTERS; SECRETION; RELEASE;
D O I：
10.1021/cn300227m
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A method for the selective labeling and imaging of catecholamines in live and fixed secretory cells is reported. The method integrates a tailored approach using a novel fluorescence-based turn-on molecular sensor (NeuroSensor 521) that can exploit the high concentration of neurotransmitters and acidic environment within secretory vesicles for the selective recognition of norepinephrine and dopamine. The utility of the method was demonstrated by selectively labeling and imaging norepinephrine in secretory vesicles such that discrimination between norepinephrine- and epinephrine-enriched populations of chromaffin cells was observed. This method was validated in fixed cells by co-staining with an anti-PNMT antibody.
引用
收藏
页码：918 / 923
页数：6
相关论文
共 33 条
  • [21] A confocal study on the visualization of chromaffin cell secretory vesicles with fluorescent targeted probes and acidic dyes
    Moreno, Alfredo
    SantoDomingo, Jaime
    Fonteriz, Rosalba I.
    Lobaton, Carmen D.
    Montero, Mayte
    Alvarez, Javier
    [J]. JOURNAL OF STRUCTURAL BIOLOGY, 2010, 172 (03) : 261 - 269
  • [22] SEPARATION AND CULTURE OF LIVING ADRENALINE-CONTAINING AND NORADRENALINE-CONTAINING CELLS FROM BOVINE ADRENAL MEDULLAE
    MORO, MA
    LOPEZ, MG
    GANDIA, L
    MICHELENA, P
    GARCIA, AG
    [J]. ANALYTICAL BIOCHEMISTRY, 1990, 185 (02) : 243 - 248
  • [23] Analysis of exocytotic events recorded by amperometry
    Mosharov, EV
    Sulzer, D
    [J]. NATURE METHODS, 2005, 2 (09) : 651 - 658
  • [24] Only a Fraction of Quantal Content is Released During Exocytosis as Revealed by Electrochemical Cytometry of Secretory Vesicles
    Omiatek, Donna M.
    Dong, Yan
    Heien, Michael L.
    Ewing, Andrew G.
    [J]. ACS CHEMICAL NEUROSCIENCE, 2010, 1 (03): : 234 - 245
  • [25] Real-Time Monitoring of Chemical Transmission in Slices of the Murine Adrenal Gland
    Petrovic, Jelena
    Walsh, Paul L.
    Thornley, Keith T.
    Miller, Charles E.
    Wightman, R. Mark
    [J]. ENDOCRINOLOGY, 2010, 151 (04) : 1773 - 1783
  • [26] Ultrastructural organization of bovine chromaffin cell cortex - Analysis by cryofixation and morphometry of aspects pertinent to exocytosis
    Plattner, H
    Artalejo, AR
    Neher, E
    [J]. JOURNAL OF CELL BIOLOGY, 1997, 139 (07) : 1709 - 1717
  • [27] Rasband WS, 1997, ImageJ
  • [28] Monitoring rapid chemical communication in the brain
    Robinson, Donita L.
    Hermans, Andre
    Seipel, Andrew T.
    Wightman, R. Mark
    [J]. CHEMICAL REVIEWS, 2008, 108 (07) : 2554 - 2584
  • [29] Selective amine recognition: Development of a chemosensor for dopamine and norepinephrine
    Secor, KE
    Glass, TE
    [J]. ORGANIC LETTERS, 2004, 6 (21) : 3727 - 3730
  • [30] Stevens D. R., 2011, Front. Synaptic Neurosci., V3, P1
1234