Engineering metabolism through dynamic control

被引：164

作者：

Venayak, Naveen ^{[1
]}

Anesiadis, Nikolaos ^{[1
]}

Cluett, William R. ^{[1
]}

Mahadevan, Radhakrishnan ^{[1
,2
]}

机构：

[1] Univ Toronto, Dept Chem Engn & Appl Chem, Toronto, ON, Canada

[2] Univ Toronto, Inst Biomat & Biomed Engn, Toronto, ON, Canada

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2015年 / 34卷

基金：

加拿大自然科学与工程研究理事会;

关键词：

ONE-STEP INACTIVATION; MESSENGER-RNA DECAY; ESCHERICHIA-COLI; GENE-EXPRESSION; GLOBAL ANALYSIS; STRAIN DESIGN; GROWTH; OPTIMIZATION; PATHWAY; RIBOSWITCHES;

D O I：

10.1016/j.copbio.2014.12.022

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Metabolic engineering has proven crucial for the microbial production of valuable chemicals. Due to the rapid development of tools in synthetic biology, there has been recent interest in the dynamic regulation of flux through metabolic pathways to overcome some of the issues arising from traditional strategies lacking dynamic control. There are many diverse implementations of dynamic control, with a range of metabolite sensors and inducers being used. Furthermore, control has been implemented at the transcriptional, translational and post-translational levels. Each of these levels have unique sets of engineering tools, and allow for control at different dynamic time-scales. In order to extend the applications of dynamic control, new tools are required to improve the dynamics of regulatory circuits. Further study and characterization of circuit robustness is also needed to improve their applicability to industry. The successful implementation of dynamic control, using technologies that are amenable to commercialization, will be a fundamental step in advancing metabolic engineering.

引用

页码：142 / 152

页数：11

共 86 条

[1]

Alon U., 2006, INTRO SYSTEMS BIOL

[2] Analysis and Design of a Genetic Circuit for Dynamic Metabolic Engineering [J].

Anesiadis, Nikolaos ;

Kobayashi, Hideki ;

Cluett, William R. ;

Mahadevan, Radhakrishnan .

ACS SYNTHETIC BIOLOGY, 2013, 2 (08) :442-452

[3] Dynamic metabolic engineering for increasing bioprocess productivity [J].

Anesiadis, Nikolaos ;

Cluett, William R. ;

Mahadevan, Radhakrishnan .

METABOLIC ENGINEERING, 2008, 10 (05) :255-266

[4]

[Anonymous], FRONT MICROBIOL

[5] Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels [J].

Atsumi, Shota ;

Hanai, Taizo ;

Liao, James C. .

NATURE, 2008, 451 (7174) :86-U13

[6] Insulating gene circuits from context by RNA processing [J].

Bashor, Caleb J. ;

Collins, James J. .

NATURE BIOTECHNOLOGY, 2012, 30 (11) :1061-1062

[7] Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays [J].

Bernstein, JA ;

Khodursky, AB ;

Lin, PH ;

Lin-Chao, S ;

Cohen, SN .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (15) :9697-9702

[8] Prospects for Riboswitch Discovery and Analysis [J].

Breaker, Ronald R. .

MOLECULAR CELL, 2011, 43 (06) :867-879

[9] OptKnock: A bilevel programming framework for identifying gene knockout strategies for microbial strain optimization [J].

Burgard, AP ;

Pharkya, P ;

Maranas, CD .

BIOTECHNOLOGY AND BIOENGINEERING, 2003, 84 (06) :647-657

[10] Synthetic RNA switches as a tool for temporal and spatial control over gene expression [J].

Chang, Andrew L. ;

Wolf, Joshua J. ;

Smolke, Christina D. .

CURRENT OPINION IN BIOTECHNOLOGY, 2012, 23 (05) :679-688

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