γ-Glutamyl-γ-aminobutyrate hydrolase in the putrescine utilization pathway of Escherichia coli K-12

被引：30

作者：

Kurihara, S ^{[1
]}

Oda, S ^{[1
]}

Kumagai, H ^{[1
]}

Suzuki, H ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Biostudies, Div Integrated Life Sci, Sakyo Ku, Kyoto 6068502, Japan

来源：

FEMS MICROBIOLOGY LETTERS | 2006年 / 256卷 / 02期

关键词：

putrescine degradation; gamma-glutamyl-gamma-aminobutyrate dehydrogenase; gamma-aminobutyrate; gamma-glutamyl compound; Escherichia coli;

D O I：

10.1111/j.1574-6968.2006.00137.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

gamma-Glutamyl-gamma-aminobutyrate hydrolase (PuuD) was purified and the properties of the enzyme were characterized. The active center of PuuD was identified as Cys-114 by site-directed mutagenesis. The expression of PuuD was induced by putrescine and O-2 (substrates of the Puu pathway), while the addition of succinate or NH4Cl (products of the Puu pathway) to the medium reduced the expression of PuuD. The findings that the puuD-deficient strain accumulated gamma-glutamyl-gamma-aminobutyrate (gamma-Glu-GABA) and could not grow on putrescine as a sole nitrogen source indicate that PuuD is physiologically important as a gamma-Glu-GABA hydrolase.

引用

页码：318 / 323

页数：6

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