Genomics-Inspired Discovery of Three Antibacterial Active Metabolites, Aurantinins B, C, and D from Compost-Associated Bacillus subtilis fmb60

被引：30

作者：

Yang, Jie ^{[1
]}

Zhu, Xiaoyu ^{[1
]}

Cao, Mingming ^{[1
]}

Wang, Changbao ^{[1
]}

Zhang, Chong ^{[1
]}

Lu, Zhaoxin ^{[1
]}

Lu, Fengxia ^{[1
]}

机构：

[1] Nanjing Agr Univ, Coll Food Sci & Technol, 1 Weigang, Nanjing 210095, Jiangsu, Peoples R China

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2016年 / 64卷 / 46期

基金：

中国国家自然科学基金;

关键词：

Bacillus subtilis; genome mining; polyketide synthase; aurantinins; bacteria control; noncytotoxicity; RESISTANT STAPHYLOCOCCUS-AUREUS; BIOCONTROL ACTIVITY; BACTERIAL ORIGIN; NATURAL-PRODUCTS; GENE CLUSTERS; BIOSYNTHESIS; ANTIBIOTICS; IDENTIFICATION; POLYKETIDE; AMYLOLIQUEFACIENS;

D O I：

10.1021/acs.jafc.6b04455

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Fmb60 is a wild-type Bacillus subtilis isolated from compost with significant broad-spectrum antimicrobial activities. Two novel PKS clusters were recognized in the genome sequence of finb60, and then three polyene antibiotics, aurantinins B, C, and D, 1-3, were obtained by bioactivity-guided isolation from the fermentation of finb60. The structures of aurantinins B-D were elucidated by LC-HRMS and NMR data analysis. Aurantinins C and D were identified as new antimicrobial compounds. The three aurantinins showed significant activity against multidrug-resistant Staphylococcus aureus and Clostridium sporogenes. However, aurantinins B-D did not exhibit any cytotoxicity (IC50 > 100 mu g/mL) against LO2 and Caco2 cell lines by MTT assay. Furthermore, using S. aureus as a model bacterium to explore the antibacterial mechanism of aurantinins B-D, it was revealed that the bactericidal activity of aurantinins B-D was related to their ability to disrupt the cell membrane.

引用

页码：8811 / 8820

页数：10

共 43 条

[1] Genome mining: Prediction of lipopeptides and polyketides from Bacillus and related Firmicutes
Aleti, Gajender
Sessitsch, Angela
Brader, Guenter
[J]. COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL, 2015, 13 : 192 - 203
[2] [Anonymous], 2011, 24 INFORMATIONAL SUP
[3] [Anonymous], 2012, Clinical and Laboratory Standards Institute: Performance Standards for Antimicrobial Disk Susceptibility Testing
[4] Twenty-Second Informational Supplement. M100-S22
[5] Table 2. Zone Diameter and MIC Interpretive Standards for Staphylococcus spp. (2C), Haemophilus influenzae and Haemophilus parainfluenzae (2E)
[6] Marine natural products
Blunt, John W.
Copp, Brent R.
Hu, Wan-Ping
Munro, Murray H. G.
Northcote, Peter T.
Prinsep, Michele R.
[J]. NATURAL PRODUCT REPORTS, 2009, 26 (02) : 170 - 244
[7] The identification of bacillaene, the product of the PksX megacomplex in Bacillus subtilis
Butcher, Rebecca A.
Schroeder, Frank C.
Fischbach, Michael A.
Straightt, Paul D.
Kolter, Roberto
Walsh, Christopher T.
Clardy, Jon
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (05) : 1506 - 1509
[8] Isolation and characterization of antagonistic Bacillus subtilis strains from the avocado rhizoplane displaying biocontrol activity
Cazorla, F. M.
Romero, D.
Pérez-García, A.
Lugtenberg, B. J. J.
de Vicente, A.
Bloemberg, G.
[J]. JOURNAL OF APPLIED MICROBIOLOGY, 2007, 103 (05) : 1950 - 1959
[9] Polyketide Family of Novel Antibacterial 7-O-Methyl-5′-hydroxy-3′-heptenoate-Macrolactin from Seaweed-Associated Bacillus subtilis MTCC 10403
Chakraborty, Kajal
Thilakan, Bini
Raola, Vamshi Krishna
[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2014, 62 (50) : 12194 - 12208
[10] Greater enhancement of Bacillus subtilis spore yields in submerged cultures by optimization of medium composition through statistical experimental designs
Chen, Zhen-Min
Li, Qing
Liu, Hua-Mei
Yu, Na
Xie, Tian-Jian
Yang, Ming-Yuan
Shen, Ping
Chen, Xiang-Dong
[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2010, 85 (05) : 1353 - 1360

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