E4ORF3 requirement for achieving long-term transgene expression from the cytomegalovirus promoter in adenovirus vectors

被引：30

作者：

Armentano, D

Smith, MP

Sookdeo, CC

Zabner, J

Perricone, MA

St George, JA

Wadsworth, SC

Gregory, RJ

机构：

[1] Genzyme Corp, Framingham, MA 01701 USA

[2] Univ Iowa, Coll Med, Howard Hughes Med Inst, Iowa City, IA 52242 USA

来源：

JOURNAL OF VIROLOGY | 1999年 / 73卷 / 08期

关键词：

D O I：

10.1128/JVI.73.8.7031-7034.1999

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Analysis of transgene expression under the control of the cytomegalovirus (CMV) promoter from adenovirus vectors in which the E4 region was modified indicated that E4ORF3 is required for long-term expression in the murine lung. CMV promoter truncation led to the persistence of expression in the absence of E4, thus eliminating the ORF3 requirement.

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页码：7031 / 7034

页数：4

相关论文

共 21 条

[1] Effect of the E4 region on the persistence of transgene expression from adenovirus vectors [J].

Armentano, D ;

Zabner, J ;

Sacks, C ;

Sookdeo, CC ;

Smith, MP ;

StGeorge, JA ;

Wadsworth, SC ;

Smith, AE ;

Gregory, RJ .

JOURNAL OF VIROLOGY, 1997, 71 (03) :2408-2416

[2] CHARACTERIZATION OF AN ADENOVIRUS GENE-TRANSFER VECTOR CONTAINING AN E4 DELETION [J].

ARMENTANO, D ;

SOOKDEO, CC ;

HEHIR, KM ;

GREGORY, RJ ;

STGEORGE, JA ;

PRINCE, GA ;

WADSWORTH, SC ;

SMITH, AE .

HUMAN GENE THERAPY, 1995, 6 (10) :1343-1353

[3] The enhancer domain of the human cytomegalovirus major immediate-early promoter determines cell type-specific expression in transgenic mice [J].

Baskar, JF ;

Smith, PP ;

Nilaver, G ;

Jupp, RA ;

Hoffmann, S ;

Peffer, NJ ;

Tenney, DJ ;

ColbergPoley, AM ;

Ghazal, P ;

Nelson, JA .

JOURNAL OF VIROLOGY, 1996, 70 (05) :3207-3214

[4] A VERY STRONG ENHANCER IS LOCATED UPSTREAM OF AN IMMEDIATE EARLY GENE OF HUMAN CYTOMEGALO-VIRUS [J].

BOSHART, M ;

WEBER, F ;

JAHN, G ;

DORSCHHASLER, K ;

FLECKENSTEIN, B ;

SCHAFFNER, W .

CELL, 1985, 41 (02) :521-530

[5] REDUNDANT CONTROL OF ADENOVIRUS LATE GENE-EXPRESSION BY EARLY REGION-4 [J].

BRIDGE, E ;

KETNER, G .

JOURNAL OF VIROLOGY, 1989, 63 (02) :631-638

[6] Activation of transgene expression by early region 4 is responsible for a high level of persistent transgene expression from adenovirus vectors in vivo [J].

Brough, DE ;

Hsu, C ;

Kulesa, VA ;

Lee, GM ;

Cantolupo, LJ ;

Lizonova, A ;

Kovesdi, I .

JOURNAL OF VIROLOGY, 1997, 71 (12) :9206-9213

[7] TARGETING OF ADENOVIRUS E1A AND E4-ORF3 PROTEINS TO NUCLEAR MATRIX-ASSOCIATED PML BODIES [J].

CARVALHO, T ;

SEELER, JS ;

OHMAN, K ;

JORDAN, P ;

PETTERSSON, U ;

AKUSJARVI, G ;

CARMOFONSECA, M ;

DEJEAN, A .

JOURNAL OF CELL BIOLOGY, 1995, 131 (01) :45-56

[8] Long-term gene delivery into the livers of immunocompetent mice with E1/E4-defective adenoviruses [J].

Dedieu, JF ;

Vigne, E ;

Torrent, C ;

Jullien, C ;

Mahfouz, I ;

Caillaud, JM ;

Aubailly, N ;

Orsini, C ;

Guillaume, JM ;

Opolon, P ;

Delaere, P ;

Perricaudet, M ;

Yeh, P .

JOURNAL OF VIROLOGY, 1997, 71 (06) :4626-4637

[9] Adenovirus replication is coupled with the dynamic properties of the PML nuclear structure [J].

Doucas, V ;

Ishov, AM ;

Romo, A ;

Juguilon, H ;

Weitzman, MD ;

Evans, RM ;

Maul, GG .

GENES & DEVELOPMENT, 1996, 10 (02) :196-207

[10] ADENOVIRUS EARLY REGION-4 ENCODES FUNCTIONS REQUIRED FOR EFFICIENT DNA-REPLICATION, LATE GENE-EXPRESSION, AND HOST-CELL SHUTOFF [J].

HALBERT, DN ;

CUTT, JR ;

SHENK, T .

JOURNAL OF VIROLOGY, 1985, 56 (01) :250-257