Enhanced optical immuosensor based on surface plasmon resonance for determination of transferrin

被引：17

作者：

Liu, X ^{[1
]}

Sun, Y ^{[1
]}

Song, DQ ^{[1
]}

Zhang, QL ^{[1
]}

Tian, Y ^{[1
]}

Zhang, HQ ^{[1
]}

机构：

[1] Jilin Univ, Coll Chem, Changchun 130012, Peoples R China

来源：

TALANTA | 2006年 / 68卷 / 03期

关键词：

SPR; wavelength modulation; transferrin; double-linker assay; colloidal Au-amplified assay;

D O I：

10.1016/j.talanta.2005.07.008

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Wavelength modulation surface plasmon resonance biosensors (SPR) using colloidal Au nanoparticles and double-linker sensing membrane enhancement are reported for determination of transferrin. The 2-mercaptoethylamine (MEA) was immobilized on the biosensor surface with traditional amine coupling method. The interaction between colloidal An nanoparticles and MEA was investigated. The anti-transferrin was immobilized on the biosensor surface prepared with staphylococcal protein A (SPA). The interaction of the antibody and antigen was monitored in real time. The good response was obtained in the concentration range 1-20, 0.1-20 and 0.05-20 mu g/mL for directly immune assay, double-linker assay and colloidal Au-amplified assay. The result clearly demonstrates that these methods may obtain significantly enhancement of sensitivity for the wavelength modulation SPR biosensor. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：1026 / 1031

页数：6

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