Enhanced production of plasmid DNA by engineered Escherichia coli strains

被引：13

作者：

Pablos, Tania E. ^{[1
]}

Soto, Rene ^{[2
]}

Meza Mora, Eugenio ^{[3
]}

Le Borgne, Sylvie ^{[1
]}

Ramirez, Octavio T. ^{[2
]}

Gosset, Guillermo ^{[3
]}

Lara, Alvaro R. ^{[1
]}

机构：

[1] Univ Autonoma Metropolitana Cuajimalpa, Dept Proc & Tecnol, Mexico City 01120, DF, Mexico

[2] Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Med Mol & Bioproc, Cuernavaca 62210, Morelos, Mexico

[3] Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Ingn Celular & Biocatalisis, Cuernavaca 62210, Morelos, Mexico

来源：

JOURNAL OF BIOTECHNOLOGY | 2012年 / 158卷 / 04期

关键词：

Plasmid DNA; Escherichia coli; PTS; pykA; pDNA vaccines; Acetate; SYSTEM; PERFORMANCE; METABOLISM; ACETATE; GLUCOSE; GROWTH;

D O I：

10.1016/j.jbiotec.2011.04.015

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Escherichia coli strains VH33 (PTS- GalP(+) strain displaying a strongly reduced overflow metabolism) and VH34 (additionally lacking the pyruvate kinase A) were evaluated for the production of a plasmid DNA (pDNA) vaccine. The parent (W3110) and mutant strains were cultured using 10 g of glucose/L. While the specific growth rates of the three strains were similar, they presented differences in the accumulation of acetate. W3110 accumulated up to 4 g/L of acetate, VH33 produced 1.4 g/L, and VH34 only 0.78 g/L. VH33 and VH34 produced 76% and 300% more pDNA than W3110. Moreover, VH34 demanded 33% less oxygen than VH33 and W3110, which can be advantageous for large-scale applications. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：211 / 214

页数：4

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