CHO Cell Line Development and Engineering via Site-specific Integration: Challenges and Opportunities

被引：26

作者：

Shin, Sung Wook ^{[1
]}

Lee, Jae Seong ^{[1
]}

机构：

[1] Ajou Univ, Dept Mol Sci & Technol, Suwon 16499, South Korea

来源：

BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | 2020年 / 25卷 / 05期

关键词：

cell line development; Chinese hamster ovary (CHO); CRISPR; Cas9; targeted integration; HAMSTER OVARY CELLS; KNOCK-IN; TARGETED INTEGRATION; HOMOLOGOUS RECOMBINATION; MAMMALIAN-CELLS; GENOME; GENE; CRISPR/CAS9; EXPRESSION; EFFICIENT;

D O I：

10.1007/s12257-020-0093-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Chinese hamster ovary (CHO) cells are utilized as primary mammalian expression host cells for the production of biopharmaceuticals. Recombinant CHO cell line development (CLD) has been a crucial step for therapeutic protein production platforms; however, this step remains time-consuming and costly. With the emergence of multiomics data sets of CHO cells and genome editing technology such as CRISPR/Cas9, site-specific integration-based cell line development, and engineering have been successfully implemented in CHO cells for predictable transgene expression and expediting the process of CLD. This review describes the trends in CHO CLD from random to targeted approaches. And we cover the major obstacles faced in rational CHO CLD and the potential strategies employed to overcome its limitations. Finally, we conclude by discussing future directions and challenges for next-generation CHO cell factories.

引用

页码：633 / 645

页数：13

共 96 条

[1] TARGETED HOMOLOGOUS RECOMBINATION AT THE ENDOGENOUS ADENINE PHOSPHORIBOSYLTRANSFERASE LOCUS IN CHINESE-HAMSTER CELLS [J].

ADAIR, GM ;

NAIRN, RS ;

WILSON, JH ;

SEIDMAN, MM ;

BROTHERMAN, KA ;

MACKINNON, C ;

SCHEERER, JB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (12) :4574-4578

[2] Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ [J].

Aida, Tomomi ;

Nakade, Shota ;

Sakuma, Tetsushi ;

Izu, Yayoi ;

Oishi, Ayu ;

Mochida, Keiji ;

Ishikubo, Harumi ;

Usami, Takako ;

Aizawa, Hidenori ;

Yamamoto, Takashi ;

Tanaka, Kohichi .

BMC GENOMICS, 2016, 17

[3] Chromatin Position Effects Assayed by Thousands of Reporters Integrated in Parallel [J].

Akhtar, Waseem ;

de Jong, Johann ;

Pindyurin, Alexey V. ;

Pagie, Ludo ;

Meuleman, Wouter ;

de Ridder, Jeroen ;

Berns, Anton ;

Wessels, Lodewyk F. A. ;

van Lohuizen, Maarten ;

van Steensel, Bas .

CELL, 2013, 154 (04) :914-927

[4] Highly efficient CRISPR/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair [J].

Auer, Thomas O. ;

Duroure, Karine ;

De Cian, Anne ;

Concordet, Jean-Paul ;

Del Bene, Filippo .

GENOME RESEARCH, 2014, 24 (01) :142-153

[5] CRISPR-Cas targeted plasmid integration into mammalian cells via non-homologous end joining [J].

Bachu, Ravichandra ;

Bergareche, Inigo ;

Chasin, Lawrence A. .

BIOTECHNOLOGY AND BIOENGINEERING, 2015, 112 (10) :2154-2162

[6] A Framework to Quantify Karyotype Variation Associated with CHO Cell Line Instability at a Single-Cell Level [J].

Baik, Jong Youn ;

Lee, Kelvin H. .

BIOTECHNOLOGY AND BIOENGINEERING, 2017, 114 (05) :1045-1053

[7] Recent advances in CRISPR/Cas9-mediated knock-ins in mammalian cells [J].

Banan, Mehdi .

JOURNAL OF BIOTECHNOLOGY, 2020, 308 :1-9

[8] A role for alternative end-joining factors in homologous recombination and genome editing in Chinese hamster ovary cells [J].

Bosshard, Sandra ;

Duroy, Pierre-Olivier ;

Mermod, Nicolas .

DNA REPAIR, 2019, 82

[9] Maximizing antibody production in a targeted integration host by optimization of subunit gene dosage and position [J].

Carver, Joe ;

Ng, Domingos ;

Zhou, Michelle ;

Ko, Peggy ;

Zhan, Dejin ;

Yim, Mandy ;

Shaw, David ;

Snedecor, Brad ;

Laird, Michael W. ;

Lang, Steven ;

Shen, Amy ;

Hu, Zhilan .

BIOTECHNOLOGY PROGRESS, 2020, 36 (04)

[10] CtIP fusion to Cas9 enhances transgene integration by homology-dependent repair [J].

Charpentier, M. ;

Khedher, A. H. Y. ;

Menoret, S. ;

Brion, A. ;

Lamribet, K. ;

Dardillac, E. ;

Boix, C. ;

Perrouault, L. ;

Tesson, L. ;

Geny, S. ;

De Cian, A. ;

Itier, J. M. ;

Anegon, I. ;

Lopez, B. ;

Giovannangeli, C. ;

Concordet, J. P. .

NATURE COMMUNICATIONS, 2018, 9

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