Highly sensitive turn-on fluorescence detection of thrombomodulin based on fluorescence resonance energy transfer

被引：6

作者：

Kong, Liyan ^{[1
]}

Zhu, Jiaming ^{[1
]}

Wang, Wen ^{[1
]}

Jin, Lehe ^{[2
]}

Fu, Yanjiao ^{[2
]}

Duan, Bohui ^{[2
]}

Tan, Liang ^{[1
,2
]}

机构：

[1] Hunan Normal Univ, Coll Chem & Chem Engn, Minist Educ China, Key Lab Chem Biol & Tradit Chinese Med Res, Changsha 410081, Hunan, Peoples R China

[2] Hunan Normal Univ, Coll Chem & Chem Engn, Dept Chem, Changsha 410081, Hunan, Peoples R China

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2017年 / 173卷

关键词：

Thrombomodulin; Immunoreaction; Acridine orange; Gold nanocomposites; Fluorescence resonance energy transfer; GOLD NANOPARTICLES; QUANTUM DOTS; TRANSFER FRET; PLASMA THROMBOMODULIN; LIVER-TRANSPLANTATION; ENDOTHELIAL DAMAGE; CARBON DOTS; BIOSENSOR; INHIBITION; IMMUNOSENSOR;

D O I：

10.1016/j.saa.2016.10.022

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

As an integral glycoprotein on the surface of endothelial cells, thrombomodulin (TM) has very high affinity for thrombin. TM has been regarded to be a marker of endothelial damage since it can be released during endothelial cell injury. In this work, a highly sensitive fluorescence method for the quantitative detection of TM was developed. TM antibody (Ab) and bovine serum albumin (BSA) were bound on gold nanoparticles (AuNPs) to construct BSA-AuNPs-Ab nanocomposites and they were characterized by transmission electron microscope and UV-vis spectrophotometry. The fluorescence of acridine orange (AO) was quenched by the prepared gold nano composites based on fluorescence resonance energy transfer (FRET). In the presence of TM, the fluorescence was turned on due to the effective separation of AO from the surface of gold nanocomposites. Under optimum conditions, the enhanced fluorescence intensity displayed a linear relationship with the logarithm of the TM concentration from 0.1 pg mL(-1) to 5 ng mL(-1) with a low detection limit of 12 fg mL(-1). The release of soluble thrombomodulin (sTM) by the injured HUVEC-C cells in the presence of H2O2 was investigated using the proposed method. The released sTM content in the growth medium was found to be increased with the enhancement of contact time of the cells with H2O2. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：675 / 680

页数：6

共 30 条

[1] Fluorescence energy transfer inhibition bioassay for cholera toxin based on galactose-stabilized gold nanoparticles and amine-terminated quantum dots [J].