Transport at the air/water interface is the reason for rings in protein microarrays

被引：89

作者：

Deng, Y

Zhu, XY

Kienlen, T

Guo, A

机构：

[1] Univ Minnesota, Dept Chem, Minneapolis, MN 55455 USA

[2] MicroSurfaces Inc, Minneapolis, MN 55421 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2006年 / 128卷 / 09期

关键词：

D O I：

10.1021/ja057669w

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Fabricating a protein microarray involves the deposition of nanoliter droplets of solutions on a solid surface. Instead of uniform spots, one often observes ring-like structures that add to the difficulty in quantification. We show that the accumulation of proteins at the air/water interface of the nanodroplet is the reason. Transformation to a uniform spot can be achieved via the addition of competitive surfactants or the control of surface reaction kinetics. Copyright © 2006 American Chemical Society.

引用

收藏

页码：2768 / 2769

页数：2

相关论文

共 12 条

[1] Next generation of protein microarray support materials:: Evaluation for protein and antibody microarray applications [J].

Angenendt, P ;

Glökler, J ;

Sobek, J ;

Lehrach, H ;

Cahill, DJ .

JOURNAL OF CHROMATOGRAPHY A, 2003, 1009 (1-2) :97-104

[2] Immobilization of oriented protein molecules on poly(ethylene glycol)-coated Si(111) [J].

Cha, T ;

Guo, A ;

Jun, Y ;

Pei, DQ ;

Zhu, XY .

PROTEOMICS, 2004, 4 (07) :1965-1976

[3] DIFFERENCES IN THE STRUCTURE AND DYNAMICS OF THE ADSORBED LAYERS IN PROTEIN-STABILIZED MODEL FOAMS AND EMULSIONS [J].

CLARK, DC ;

MACKIE, AR ;

WILDE, PJ ;

WILSON, DR .

FARADAY DISCUSSIONS, 1994, 98 :253-262

[4] Capillary flow as the cause of ring stains from dried liquid drops [J].

Deegan, RD ;

Bakajin, O ;

Dupont, TF ;

Huber, G ;

Nagel, SR ;

Witten, TA .

NATURE, 1997, 389 (6653) :827-829

[5] Methods and applications of antibody microarrays in cancer research [J].

Haab, BB .

PROTEOMICS, 2003, 3 (11) :2116-2122

[6] Cylinder alignment in annular structures of microphase-separated polystyrene-b-poly(methyl methacrylate) [J].

Hahm, J ;

Sibener, SJ .

LANGMUIR, 2000, 16 (11) :4766-4769

[7]

HANASH S, 2003, PROTEOMICS, P3

[8] Peptide chips for the quantitative evaluation of protein kinase activity [J].

Houseman, BT ;

Huh, JH ;

Kron, SJ ;

Mrksich, M .

NATURE BIOTECHNOLOGY, 2002, 20 (03) :270-274

[9]

MacBeath G, 2000, SCIENCE, V289, P1760

[10] Orogenic displacement of protein from the air/water interface by competitive adsorption [J].

Mackie, AR ;

Gunning, AP ;

Wilde, PJ ;

Morris, VJ .

JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1999, 210 (01) :157-166