Long term cryopreservation in 5% DMSO maintains unchanged CD34+ cells viability and allows satisfactory hematological engraftment after peripheral blood stem cell transplantation

被引：18

作者：

Abbruzzese, L. ^{[1
]}

Agostini, F. ^{[1
]}

Durante, C. ^{[1
]}

Toffola, R. T. ^{[1
]}

Rupolo, M. ^{[2
]}

Rossi, F. M. ^{[3
]}

Lleshi, A. ^{[2
]}

Zanolin, S. ^{[1
]}

Michieli, M. ^{[2
]}

Mazzucato, M. ^{[1
]}

机构：

[1] IRCCS, Ctr Riferimento Oncol, Dipartimento Lab Diagnost & Terapie Cellulari, Unita Raccolta & Manipolaz Cellule Staminali, Aviano, Italy

[2] IRCCS, Ctr Riferimento Oncol, Dipartimento Oncol Med, Unita Terapia Cellulare & Alte Dosi, Aviano, Italy

[3] IRCCS, Ctr Riferimento Oncol, Unita Oncoematol Clin & Sperimentale, Dipartimento Lab Diagnost & Terapie Cellulari, Aviano, Italy

来源：

VOX SANGUINIS | 2013年 / 105卷 / 01期

关键词：

5% DMSO; CD34+viability; engraftment; EVA-teflon double bags; long-term cryopreservation; PROGENITOR CELLS; DIMETHYL-SULFOXIDE; HEMATOPOIETIC STEM;

D O I：

10.1111/vox.12012

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Peripheral blood stem cell cryopreservation is associated with cell damage and decreased viability. We evaluated the impact of up to 10years of cryopreservation (5% DMSO) on viability of CD34+ cells utilizing graft samples of consecutive patients (2002-2012) with different malignancies who underwent stem cell collection and transplantation. Viability of CD34+ cells from oncohaematological patients measured after 5weeks (97 center dot 2 +/- 0 center dot 6%) or after 9-10years of cryopreservation (95 center dot 9 +/- 0 center dot 5%) was unaffected. Haemoglobin, granulocyte and platelet recovery after transplantation of long-term cryopreserved grafts occurred within 8-13days. CD34+ stem cells can be safely stored up to 9-10years, without affecting cell viability and clinical effectiveness.

引用

页码：77 / 80

页数：4

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