Ultrasensitive electrochemical Ochratoxin A aptasensor based on CdTe quantum dots functionalized graphene/Au nanocomposites and magnetic separation

被引：49

作者：

Hao, Nan ^{[1
]}

Jiang, Ling ^{[1
]}

Qian, Jing ^{[1
]}

Wang, Kun ^{[1
,2
]}

机构：

[1] Jiangsu Univ, Sch Chem & Chem Engn, Zhenjiang 212013, Peoples R China

[2] Jiangsu Univ, Inst Agr Engn, Key Lab Modern Agr Equipment & Technol, Zhenjiang 212013, Peoples R China

来源：

JOURNAL OF ELECTROANALYTICAL CHEMISTRY | 2016年 / 781卷

基金：

中国国家自然科学基金;

关键词：

Ochratoxin A; Aptamer; Nanocomposites; Electrochemistry; LABEL-FREE; GOLD NANOPARTICLES; SENSITIVE DETECTION; APTAMER; AMPLIFICATION; ELECTRODE; NANOHYBRIDS; BIOSENSOR; THROMBIN; REMOVAL;

D O I：

10.1016/j.jelechem.2016.09.053

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Ochratoxin A (OTA) is one of the most toxic food contaminant mycotoxin, which can cause nephrotoxicity, teratogenicity, and carcinogenicity activity. Here an ultrasensitive electrochemical aptasensor for OTA was successfully fabricated. This aptasensor was constructed by combining nanocomposites of gold nanoparticles (AuNPs) functionalized silica-coated iron oxide magnetic nanoparticles (mSiO(2)@Au) and cadmium telluride quantum dots (CdTe QDs) modified graphene/AuNPs nanocomposites (GAu/CdTe). The as-prepared aptasensor exhibited a wide linear range (0.2 pg mL(-1) -4 ng mL(-1)), and an extremely low detection limit of 0.07 pg m(L-1) (S/N = 3). This work provides a novel strategy for the ultrasensitive detection of various target molecules and would have great potential in food safety monitoring and clinical diagnosis. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：332 / 338

页数：7

共 41 条

[1] Immunotoxic effects of Ochratoxin A in wistar rats after oral administration [J].

Alvarez, L ;

Gil, AG ;

Ezpeleta, O ;

García-Jalón, JA ;

de Cerain, AL .

FOOD AND CHEMICAL TOXICOLOGY, 2004, 42 (05) :825-834

[2] Electrochemistry of Graphene and Related Materials [J].

Ambrosi, Adriano ;

Chua, Chun Kiang ;

Bonanni, Alessandra ;

Pumera, Martin .

CHEMICAL REVIEWS, 2014, 114 (14) :7150-7188

[3] A simple and rapid biosensor for ochratoxin A based on a structure-switching signaling aptamer [J].

Chen, Junhua ;

Fang, Zhiyuan ;

Liu, Jie ;

Zeng, Lingwen .

FOOD CONTROL, 2012, 25 (02) :555-560

[4] Aptamer-conjugated bio-bar-code Au-Fe3O4 nanoparticles as amplification station for electrochemiluminescence detection of tumor cells [J].

Chen, Min ;

Bi, Sai ;

Jia, Xiaoqiang ;

He, Peng .

ANALYTICA CHIMICA ACTA, 2014, 837 :44-51

[5] A colorimetric aptamer biosensor based on cationic polymer and gold nanoparticles for the ultrasensitive detection of thrombin [J].

Chen, Zhengbo ;

Tan, Yuan ;

Zhang, Chenmeng ;

Yin, Lu ;

Ma, He ;

Ye, Nengsheng ;

Qiang, Hong ;

Lin, Yuqing .

BIOSENSORS & BIOELECTRONICS, 2014, 56 :46-50

[6] A review on the occurrence and control of ochratoxigenic fungal species and ochratoxin A in dehydrated grapes, non-fortified dessert wines and dried vine fruit in the Mediterranean area [J].

Covarelli, L. ;

Beccari, G. ;

Marini, A. ;

Tosi, L. .

FOOD CONTROL, 2012, 26 (02) :347-356

[7] ULTRAVIOLET VISIBLE ABSORPTION-SPECTRA OF THE COLLOIDAL METALLIC ELEMENTS [J].

CREIGHTON, JA ;

EADON, DG .

JOURNAL OF THE CHEMICAL SOCIETY-FARADAY TRANSACTIONS, 1991, 87 (24) :3881-3891

[8] Fluorescence Polarization Based Displacement Assay for the Determination of Small Molecules with Aptamers [J].

Cruz-Aguado, Jorge A. ;

Penner, Gregory .

ANALYTICAL CHEMISTRY, 2008, 80 (22) :8853-8855

[9] A near-infrared magnetic aptasensor for Ochratoxin A based on near-infrared upconversion nanoparticles and magnetic nanoparticles [J].

Dai, Shaoliang ;

Wu, Shijia ;

Duan, Nuo ;

Wang, Zhouping .

TALANTA, 2016, 158 :246-253

[10] Label-Free Sensitive Electrogenerated Chemiluminescence Aptasensing Based on Chitosan/Ru(bpy)32+/Silica Nanoparticles Modified Electrode [J].

Dang, Jie ;

Guo, Zhihui ;

Zheng, Xingwang .

ANALYTICAL CHEMISTRY, 2014, 86 (18) :8943-8950

← 1 2 3 4 5 →