Naturally occurring glucagon-like peptide-2 (GLP-2) receptors in human intestinal cell lines

被引：8
作者：
Sams, A
Hastrup, S
Andersen, M
Thim, L
机构：
[1] Novo Nordisk AS, Dept Mol Pharmacol, DK-2760 Malov, Denmark
[2] Novo Nordisk AS, Dept Microbial Cell Technol, DK-2760 Malov, Denmark
[3] Novo Nordisk AS, Dept Prot Engn, DK-2760 Malov, Denmark
来源：
EUROPEAN JOURNAL OF PHARMACOLOGY | 2006年 / 532卷 / 1-2期
关键词：
GLP-2; receptor; G alpha protein signalling; intestinal growth; RT-PCR; western blot; cAMP;
D O I：
10.1016/j.ejphar.2005.12.001
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Although clinical trials with GLP-2 receptor agonists are currently ongoing, the mechanisms behind GLP-2-induced intestinal epithelial growth remain to be understood. To approach the GLP-2 mechanism of action this study aimed to identify intestinal cell lines endogenously expressing the GLP-2 receptor. Here we report the first identification of a cell line endogenously expressing functional GLP-2 receptors. The human intestinal epithelial cell line, FHC, expressed GLP-2 receptor encoding mRNA (RT-PCR) and GLP-2 receptor protein (Western blot). In cultured FHC cells, GLP-2 induced concentration dependent cAMP accumulation (pEC50=9.7 +/- 0.04 (mean S.E.M., n=4)). In addition, a naturally occurring human intestinal fibroblast cell line, 18Co, endogenously expressing GLP-2 receptor encoding mRNA (RTPCR) and protein (Western blot) was identified. No receptor functionality (binding or G-protein signalling) could be demonstrated in 18Co cells. The identified gut-relevant cell lines provide tools for future clarification of the mechanisms underlying GLP-2-induced epithelial growth. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码：18 / 23
页数：6
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