Microsatellite markers in Triatoma pseudomaculata (Hemiptera, Reduviidae, Triatominae), Chagas' disease vector in Brazil

被引:10
作者
Harry, M. [1 ]
Dupont, L. [1 ]
Romana, C. [2 ]
Demanche, C. [1 ]
Mercier, A. [1 ]
Livet, A. [1 ]
Diotaiuti, L. [3 ]
Noireau, F. [4 ]
Emperaire, L. [4 ]
机构
[1] Univ Paris 12, UFR Sci, UMR 137, F-94000 Creteil, France
[2] Univ Paris 05, IRD, Unite Espace, Montpellier, France
[3] Fiocruz MS, Ctr Pesquisas Rene Rachou, Fundacao Oswaldo Cruz, Lab Triatomineos & Epidemiol Doenca Chagas, BR-30190 Belo Horizonte, MG, Brazil
[4] IRD, UR016, Montpellier, France
关键词
Triatoma pseudomaculata; Chagas' disease vector; Microsatellite; Cross-amplification; Brazil;
D O I
10.1016/j.meegid.2008.03.005
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Six polymorphic microsatellite loci were isolated and characterized using a microsatellite-enriched genomic library from the Chagas' disease vector Triatoma pseudomaculata. This species is found in Brasil in Caatinga areas and predominantly in peridomestic habitats. All the microsatellites tested on a population of T. pseudomaculata sampled in the Bahia State, Brazil, were polymorphic (2-15 alleles). Markers amplification was also tested on six Triatoma species and some loci successfully amplified in the most phylogenetically related species, in particular Triatoma brasiliensis. (C) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码:672 / 675
页数:4
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