Sperm SPACA6 protein is required for mammalian Sperm-Egg Adhesion/Fusion

被引：60

作者：

Barbaux, Sandrine ^{[1
]}

Ialy-Radio, Come ^{[1
]}

Chalbi, Myriam ^{[1
]}

Dybal, Elisa ^{[1
]}

Homps-Legrand, Meline ^{[1
]}

Do Cruzeiro, Marcio ^{[1
]}

Vaiman, Daniel ^{[1
]}

Wolf, Jean-Philippe ^{[1
,2
]}

Ziyyat, Ahmed ^{[1
,2
]}

机构：

[1] Univ Paris, Inst Cochin, INSERM, CNRS, F-75014 Paris, France

[2] Hop Cochin, AP HP, Serv Histol Embryol Biol Reprod, F-75014 Paris, France

来源：

SCIENTIFIC REPORTS | 2020年 / 10卷 / 01期

关键词：

GAMETE FUSION; IZUMO1; CD9; OOCYTE; JUNO; MEMBRANE; RECEPTOR; FERTILIZATION;

D O I：

10.1038/s41598-020-62091-y

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Three genes are known to be essential for gamete adhesion/fusion (Cd9, Izumo1 and Juno). Here, we confirmed that Spaca6 null males are infertile and showed that their sperm accumulate in the perivitelline space but are unable to fuse with oocyte. Like IZUMO1, SPACA6 which is expressed by human sperm, is remained on the equatorial segment after acrosomal reaction and is involved in human fertilization since an anti-SPACA6 antibody inhibited it. Despite the similarity of the phenotypes caused by Spaca6 and Izumo1 knockouts, these are not redundant and the essential relocation of IZUMO1 is not affected by the lack of SPACA6. We propose a model in which IZUMO1 and SPACA6 would be part of a molecular complex necessary for gamete fusion and that their concomitant presence would be required for the recruitment of another essential molecular actor, such as a fusogen, for the fusion to take place.

引用

页数：15

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