Oligosaccharide analysis by capillary-scale high-pH anion-exchange chromatography with on-line ion-trap mass spectrometry

被引：56
作者：
Bruggink, C
Wuhrer, M
Koeleman, CAM
Barreto, V
Liu, Y
Pohl, C
Ingendoh, A
Hokke, CH
Deelder, AM
机构：
[1] Leiden Univ, Med Ctr, Ctr Infect Dis, Dept Parasitol,Biomol Mass Spectrometry Unit, NL-2300 RC Leiden, Netherlands
[2] Dionex Chem Corp, Sunnyvale, CA 94088 USA
[3] Dionex BV, NL-4854 MB Bavel, Netherlands
[4] Bruker Daltonik GmbH, D-28359 Bremen, Germany
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2005年 / 829卷 / 1-2期
关键词：
HPAEC-PAD; inulin; G(M1)-gangliosidosis; on-line desalter; clinical glycomics;
D O I：
10.1016/j.jchromb.2005.10.006
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A capillary-scale high-pH anion-exchange chromatography (HPAEC) system for the analysis of carbohydrates was developed, in combination with two parallel on-line detection methods of sub-picomolar sensitivity: (1) pulsed amperometric detection (PAD); (2) capillary-scale desalting followed by electrospray ion-trap (IT) mass spectrometry (MS). The capillary chromatographic system combined the superb selectivity of HPAEC that allows routine separation of isomeric oligosaccharides with the information on monosaccharide sequence and linkage positions obtained by MS/MS fragmentation using the IT-MS. The applicability of the system in biomedical research was demonstrated by its use for the analysis of a urine sample of a G(M1)-gangliosidosis patient. Isomeric glycans in the sample could be resolved by HPAEC and assigned on the basis of the monosaccharide linkage information revealed by on-line IT-MS/MS. (c) 2005 Elsevier B.V. All rights reserved.
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收藏
页码：136 / 143
页数：8
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