Spectral components of bioluminescence of aequorin and obelin

被引：32

作者：

Belogurova, Nadezhda V. ^{[1
,2
]}

Kudryasheva, Nadezhda S. ^{[1
,2
]}

Alieva, Rosa R. ^{[2
]}

Sizykh, Arnold G. ^{[2
]}

机构：

[1] Inst Biophys SB RAS, Krasnoyarsk 660036, Russia

[2] Siberian Fed Univ, Krasnoyarsk 660043, Russia

来源：

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY | 2008年 / 92卷 / 02期

关键词：

bioluminescence; photoprotein; spectral components; proton transfer;

D O I：

10.1016/j.jphotobiol.2008.05.006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Complex bioluminescence spectra of photoproteins from marine coelenterates - jellyfish Aequorea victoria and hydroid Obelia longissima, and photoluminescence spectra of the bioluminescent reaction products (Ca2+-discharged photoproteins) were deconvolved into components. The bioluminescence spectra of aequorin were found to include three, the bioluminescence spectra of obelin - four, and the photoluminescence spectra of the Ca2+-discharged photoproteins - only two components. The spectral components were assigned to one unionized and three ionized forms of coelenteramide. The changes in acidity of the excited coelenteramide molecule are discussed. The differences in bioluminescence and photoluminescence spectra are considered, with protonic environment of coelenteramide taken into account. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：117 / 122

页数：6

共 48 条

[1] MEASUREMENT OF CA-2+ CONCENTRATIONS IN LIVING CELLS [J].

BLINKS, JR ;

WIER, WG ;

HESS, P ;

PRENDERGAST, FG .

PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY, 1982, 40 (1-2) :1-114

[2]

Bondar' V. S., 1992, Biokhimiya, V57, P1481

[3] EVIDENCE FOR SIMILAR BIOCHEMICAL REQUIREMENTS FOR BIOLUMINESCENCE AMONG COELENTERATES [J].

CORMIER, MJ ;

HORI, K ;

KARKHANIS, YD ;

ANDERSON, JM ;

WAMPLER, JE ;

MORIN, JG ;

HASTINGS, JW .

JOURNAL OF CELLULAR PHYSIOLOGY, 1973, 81 (02) :291-297

[4] CLONING, EXPRESSION AND SEQUENCE-ANALYSIS OF CDNA FOR THE CA2+-BINDING PHOTOPROTEIN, MITROCOMIN [J].

FAGAN, TF ;

OHMIYA, Y ;

BLINKS, JR ;

INOUYE, S ;

TSUJI, FI .

FEBS LETTERS, 1993, 333 (03) :301-305

[5]

Frundzhyan VG, 1999, APPL BIOCHEM MICRO+, V35, P321

[6]

HASTINGS JW, 2001, BIOLUMINESCENCE CELL, V3, P1115

[7] The crystal structure of the photoprotein aequorin at 2.3 Å resolution [J].

Head, JF ;

Inouye, S ;

Teranishi, K ;

Shimomura, O .

NATURE, 2000, 405 (6784) :372-376

[8] Bioluminescent properties of fluorinated semi-synthetic aequorins [J].

Hirano, T ;

Ohmiya, Y ;

Maki, S ;

Niwa, H ;

Ohashi, M .

TETRAHEDRON LETTERS, 1998, 39 (31) :5541-5544

[9]

HIRANO TT, 1994, CHEM COMMUN, V2, P165

[10] SEQUENCE OF THE CDNA-ENCODING THE CA2+-ACTIVATED PHOTOPROTEIN OBELIN FROM THE HYDROID POLYP OBELIA-LONGISSIMA [J].

ILLARIONOV, BA ;

BONDAR, VS ;

ILLARIONOVA, VA ;

VYSOTSKI, ES .

GENE, 1995, 153 (02) :273-274

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