Integrated pneumatic micro-pumps for high-throughput droplet-based microfluidics

被引:18
作者
Choi, Jae-Won [1 ]
Lee, Sangmin [2 ]
Lee, Dong-Hun [3 ]
Kim, Joonwon [2 ]
deMello, Andrew J. [4 ]
Chang, Soo-Ik [1 ]
机构
[1] Chungbuk Natl Univ, Dept Biochem, Chongju 361763, South Korea
[2] Pohang Univ Sci & Technol, Dept Mech Engn, Pohang 790784, South Korea
[3] Chungbuk Natl Univ, Dept Microbiol, Chongju 361763, South Korea
[4] ETH, Dept Chem & Appl Biosci, CH-8093 Zurich, Switzerland
基金
新加坡国家研究基金会;
关键词
PROTEIN-PROTEIN INTERACTIONS; ENHANCED RAMAN-SCATTERING; ANGIOGENIN; CHEMISTRY;
D O I
10.1039/c4ra02033b
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Droplet-based microfluidic systems have recently emerged as powerful experimental tools in the chemical and biological sciences. In conventional droplet-based microfluidics, controlled droplet generation is normally achieved using precision syringe pumps, where the sample is delivered to the microdevice using external tubing that possesses an appreciable dead volume. Accordingly, there is an unmet need for a droplet generation system that does not require the use of syringe pumps. Herein, we report the integration of pneumatic micro-pumps with droplet-based microfluidic systems and their subsequent use in high-throughput biological experimentation. The efficacy of the system is demonstrated by investigating the interaction and the binding inhibition between angiogenin and the anti-angiogenin antibody with a dissociation constant (K-D) value of 9.1 +/- 3.5 nM and a half maximal inhibitory concentration (IC50) value of 12.2 +/- 2.5 nM, respectively.
引用
收藏
页码:20341 / 20345
页数:5
相关论文
共 28 条
  • [1] Integrated on-chip derivatization and electrophoresis for the rapid analysis of biogenic amines
    Beard, NP
    Edel, JB
    deMello, AJ
    [J]. ELECTROPHORESIS, 2004, 25 (14) : 2363 - 2373
  • [2] Droplet microfluidic technology for single-cell high-throughput screening
    Brouzes, Eric
    Medkova, Martina
    Savenelli, Neal
    Marran, Dave
    Twardowski, Mariusz
    Hutchison, J. Brian
    Rothberg, Jonathan M.
    Link, Darren R.
    Perrimon, Norbert
    Samuels, Michael L.
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (34) : 14195 - 14200
  • [3] Mapping of Fluidic Mixing in Microdroplets with 1 μs Time Resolution Using Fluorescence Lifetime Imaging
    Solvas, Xavier Casadevall i
    Srisa-Art, Monpichar
    demello, Andrew J.
    Edel, Joshua B.
    [J]. ANALYTICAL CHEMISTRY, 2010, 82 (09) : 3950 - 3956
  • [4] Detection, quantitation, and localization of bovine angiogenin by immunological assays
    Chang, SI
    Jeong, GB
    Park, SH
    Ahn, BC
    Choi, JD
    Chae, Q
    Namgoong, SK
    Chung, SI
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1997, 232 (02) : 323 - 327
  • [5] Recent advances in microfluidic technologies for biochemistry and molecular biology
    Cho, Soongwon
    Kang, Dong-Ku
    Choo, Jaebum
    deMello, Andrew J.
    Chang, Soo-Ik
    [J]. BMB REPORTS, 2011, 44 (11) : 705 - 712
  • [6] High-Throughput Analysis of Protein-Protein Interactions in Picoliter-Volume Droplets Using Fluorescence Polarization
    Choi, Jae-Won
    Kang, Dong-Ku
    Park, Hyun
    deMello, Andrew J.
    Chang, Soo-Ik
    [J]. ANALYTICAL CHEMISTRY, 2012, 84 (08) : 3849 - 3854
  • [7] On-Chip Immunoassay Using Surface-Enhanced Raman Scattering of Hollow Gold Nanospheres
    Chon, Hyangah
    Lim, Chaesung
    Ha, Seung-Mo
    Ahn, Yoomin
    Lee, Eun Kyu
    Chang, Soo-Ik
    Seong, Gi Hun
    Choo, Jaebum
    [J]. ANALYTICAL CHEMISTRY, 2010, 82 (12) : 5290 - 5295
  • [8] Functional single-cell hybridoma screening using droplet-based microfluidics
    El Debs, Bachir
    Utharala, Ramesh
    Balyasnikova, Irina V.
    Griffiths, Andrew D.
    Merten, Christoph A.
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2012, 109 (29) : 11570 - 11575
  • [9] Fan SK, 2011, LAB CHIP, V11, P343, DOI [10.1039/c0lc00178c, 10.1039/c01c00178c]
  • [10] Highly sensitive trace analysis of paraquat using a surface-enhanced Raman scattering microdroplet sensor
    Gao, Rongke
    Choi, Namhyun
    Chang, Soo-Ik
    Kang, Seong Ho
    Song, Joon Myong
    Cho, Seong In
    Lim, Dong Woo
    Choo, Jaebum
    [J]. ANALYTICA CHIMICA ACTA, 2010, 681 (1-2) : 87 - 91