MESENCHYMAL STEM CELLS ISOLATED FROM HUMAN PERIODONTAL LIGAMENT

被引：21
作者：
Miletic, Maja [1 ]
Mojsilovic, S. [2 ]
Okic-Dordevic, Ivana [2 ]
Kukolj, Tamara [2 ]
Jaukovic, Aleksandra [2 ]
Santibanez, J. F. [2 ]
Jovcic, Gordana [2 ]
Bugarski, Diana [2 ]
机构：
[1] Univ Belgrade, Fac Med Dent, Belgrade 11000, Serbia
[2] Univ Belgrade, Inst Med Res, Lab Expt Hematol & Stem Cells, Belgrade 11000, Serbia
来源：
ARCHIVES OF BIOLOGICAL SCIENCES | 2014年 / 66卷 / 01期
关键词：
Periodontal ligament; mesenchymal stem cells; characterization; proliferation; differentiation; tissue regeneration; THERAPY POSITION STATEMENT; UMBILICAL-CORD BLOOD; BONE-MARROW; INTERNATIONAL-SOCIETY; REGENERATION; TISSUE; MODEL; DIFFERENTIATION; TRANSPLANTATION;
D O I：
10.2298/ABS1401261M
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Mesenchymal stem cells (MSCs) were isolated from human periodontal ligament (hPDL-MSCs) and characterized by their morphology, clonogenic efficiency, proliferation and differentiation capabilities. hPDL-MSCs, derived from normal impacted third molars, possessed all of the properties of MSC, including clonogenic ability, high proliferation rate and multi-lineage (osteogenic, chondrogenic, adipogenic, myogenic) differentiation potential. Moreover, hPDL-MSCs expressed a typical MSC epitope profile, being positive for mesenchymal cell markers (CD44H, CD90, CD105, CD73, CD29, Stro-1, fibronectin, vimentin, alpha-SMA), and negative for hematopoietic stem cell markers (CD34, CD11b, CD45, Glycophorin-CD235a). Additionally, hPDL-MSCs, as primitive and highly multipotent cells, showed high expression of embryonic markers (Nanog, Sox2, SSEA4). The data obtained provided yet further proof that cells with mesenchymal properties can be obtained from periodontal ligament tissue. Although these cells should be further investigated to determine their clinical significance, hPDL-MSCs are believed to provide a renewable and promising cell source for new therapeutic strategies in the treatment of periodontal defects.
引用
收藏
页码：261 / 271
页数：11
相关论文
共 35 条
[1]   Application of periodontal ligament cell sheet for periodontal regeneration: a pilot study in beagle dogs [J].
Akizuki, T ;
Oda, S ;
Komaki, M ;
Tsuchioka, H ;
Kawakatsu, N ;
Kikuchi, A ;
Yamato, M ;
Okano, T ;
Ishikawa, I .
JOURNAL OF PERIODONTAL RESEARCH, 2005, 40 (03) :245-251
[2]  
Armitage G.C., 2009, JADA, V140, P365
[3]   Mesenchymal stem cells: Revisiting history, concepts, and assays [J].
Bianco, Paolo ;
Robey, Pamela Gehron ;
Simmons, Paul J. .
CELL STEM CELL, 2008, 2 (04) :313-319
[4]   The tumorigenicity of human embryonic stem cells [J].
Blum, Barak ;
Benvenisty, Nissim .
ADVANCES IN CANCER RESEARCH, VOL 100, 2008, 100 :133-+
[5]   MESENCHYMAL STEM-CELLS [J].
CAPLAN, AI .
JOURNAL OF ORTHOPAEDIC RESEARCH, 1991, 9 (05) :641-650
[6]   A review on endogenous regenerative technology in periodontal regenerative medicine [J].
Chen, Fa-Ming ;
Zhang, Jing ;
Zhang, Min ;
An, Ying ;
Chen, Fang ;
Wu, Zhi-Fen .
BIOMATERIALS, 2010, 31 (31) :7892-7927
[7]   Allogeneic Periodontal Ligament Stem Cell Therapy for Periodontitis in Swine [J].
Ding, Gang ;
Liu, Yi ;
Wang, Wei ;
Wei, Fulan ;
Liu, Dayong ;
Fan, Zhipeng ;
An, Yunqing ;
Zhang, Chunmei ;
Wang, Songlin .
STEM CELLS, 2010, 28 (10) :1829-1838
[8]   Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement [J].
Dominici, M. ;
Le Blanc, K. ;
Mueller, I. ;
Slaper-Cortenbach, I. ;
Marini, F. C. ;
Krause, D. S. ;
Deans, R. J. ;
Keating, A. ;
Prockop, D. J. ;
Horwitz, E. M. .
CYTOTHERAPY, 2006, 8 (04) :315-317
[9]   Utility of PDL progenitors for in vivo tissue regeneration: a report of 3 cases [J].
Feng, F. ;
Akiyama, K. ;
Liu, Y. ;
Yamaza, T. ;
Wang, T-M ;
Chen, J-H ;
Wang, B. B. ;
Huang, G. T-J ;
Wang, S. ;
Shi, S. .
ORAL DISEASES, 2010, 16 (01) :20-28
[10]   Isolation and characterization of multipotent human periodontal ligament stem cells [J].
Gay, I. C. ;
Chen, S. ;
MacDougall, M. .
ORTHODONTICS & CRANIOFACIAL RESEARCH, 2007, 10 (03) :149-160
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