Insect Feeding Assays with Spodoptera exigua on Arabidopsis thaliana

被引:4
|
作者
You, Yanrong [1 ,2 ]
An, Chunpeng [1 ,2 ]
Li, Chuanyou [1 ,2 ]
机构
[1] Chinese Acad Sci, Innovat Acad Seed Design, Natl Ctr Plant Gene Res Beijing, Inst Genet & Dev Biol,State Key Lab Plant Genom, Beijing 100101, Peoples R China
[2] Univ Chinese Acad Sci, CAS Ctr Excellence Biot Interact, Beijing 100049, Peoples R China
来源
BIO-PROTOCOL | 2020年 / 10卷 / 05期
基金
中国国家自然科学基金;
关键词
Plant-insect interaction; chewing insects; Spodoptera exigua; Arabidopsis thaliana; Insect feeding assay;
D O I
10.21769/BioProtoc.3538
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Plant-insect interaction is an important field for studying plant immunity. The beet armyworm, Spodoptera exigua, is one of the best-known agricultural pest insects and is usually used to study plant interactions with chewing insects. Here, we describe a protocol for insect feeding assays with Spodoptera exigua lavae using model host plant Arabidopsis thaliana, which is simple and easy to conduct, and can be used to evaluate the effect of host genes on insect growth and thus to study plant resistance to chewing insects.
引用
收藏
页数:8
相关论文
共 50 条
  • [41] FEEDING AND DISPERSION OF SPODOPTERA-EXIGUA LARVAE AND ITS RELEVANCE FOR CONTROL WITH A NUCLEAR POLYHEDROSIS-VIRUS
    SMITS, PH
    VANVELDEN, MC
    VANDEVRIE, M
    VLAK, JM
    ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA, 1987, 43 (01) : 67 - 72
  • [42] Pupation behaviour, depth, and site of Spodoptera exigua
    Zheng, Xia-Lin
    Cong, Xiao-Ping
    Wang, Xiao-Ping
    Lei, Chao-Liang
    BULLETIN OF INSECTOLOGY, 2011, 64 (02): : 209 - 214
  • [43] Cold hardiness in Spodoptera exigua (Lepidoptera: Noctuidae)
    Kim, Y
    Kim, N
    ENVIRONMENTAL ENTOMOLOGY, 1997, 26 (05) : 1117 - 1123
  • [44] Action of major insecticide groups on insect cell lines of the beet armyworm, Spodoptera exigua, compared with larvicidal toxicity
    Decombel, L
    Smagghe, G
    Tirry, L
    IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL, 2004, 40 (1-2) : 43 - 51
  • [45] Action of major insecticide groups on insect cell lines of the beet armyworm, Spodoptera exigua, compared with larvicidal toxicity
    Luc Decombel
    Guy Smagghe
    Luc Tirry
    In Vitro Cellular & Developmental Biology - Animal, 2004, 40 : 43 - 51
  • [46] Ten Widely Used Canola Genotypes: A Complex of Highly Resistant and Susceptible Hosts to Feeding Spodoptera exigua
    Fathipour, Y.
    Pourghasem, M.
    Bagheri, A.
    Talebi, A. A.
    JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY, 2020, 22 (06): : 1511 - 1522
  • [47] Function of Spodoptera exigua nucleopolyhedrovirus late gene expression factors in the insect cell line SF-21
    Berretta, Marcelo F.
    Passarelli, A. Lorena
    VIROLOGY, 2006, 355 (01) : 82 - 93
  • [48] Joint Toxicity of Beauveria bassiana and Two Insect Growth Regulators on Beet Armyworm,Spodoptera exigua(Hübner)
    Hu Anling
    Tian Zhaofeng
    Gao Xiwu
    Nong Xiangqun
    Li Yongdan
    Plant Diseases and Pests, 2016, (Z1) : 1 - 4
  • [49] Arabidopsis-Green Peach Aphid Interaction: Rearing the Insect, No-choice and Fecundity Assays, and Electrical Penetration Graph Technique to Study Insect Feeding Behavior
    Nalam, Vamsi
    Louise, Joe
    Patel, Monika
    Shah, Jyoti
    BIO-PROTOCOL, 2018, 8 (15):
  • [50] Hemolin, an immunoglobulin-like peptide, opsonizes nonself targets for phagocytosis and encapsulation in Spodoptera exigua, a lepidopteran insect
    Jung, Jihyeon
    Sajjadian, Seyede Minoo
    Kim, Yonggyun
    JOURNAL OF ASIA-PACIFIC ENTOMOLOGY, 2019, 22 (03) : 947 - 956