Insect Feeding Assays with Spodoptera exigua on Arabidopsis thaliana

被引:4
|
作者
You, Yanrong [1 ,2 ]
An, Chunpeng [1 ,2 ]
Li, Chuanyou [1 ,2 ]
机构
[1] Chinese Acad Sci, Innovat Acad Seed Design, Natl Ctr Plant Gene Res Beijing, Inst Genet & Dev Biol,State Key Lab Plant Genom, Beijing 100101, Peoples R China
[2] Univ Chinese Acad Sci, CAS Ctr Excellence Biot Interact, Beijing 100049, Peoples R China
来源
BIO-PROTOCOL | 2020年 / 10卷 / 05期
基金
中国国家自然科学基金;
关键词
Plant-insect interaction; chewing insects; Spodoptera exigua; Arabidopsis thaliana; Insect feeding assay;
D O I
10.21769/BioProtoc.3538
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Plant-insect interaction is an important field for studying plant immunity. The beet armyworm, Spodoptera exigua, is one of the best-known agricultural pest insects and is usually used to study plant interactions with chewing insects. Here, we describe a protocol for insect feeding assays with Spodoptera exigua lavae using model host plant Arabidopsis thaliana, which is simple and easy to conduct, and can be used to evaluate the effect of host genes on insect growth and thus to study plant resistance to chewing insects.
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页数:8
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