Pros and Cons of In Vitro Methods for Circular RNA Preparation

被引：24

作者：

Lee, Kyung Hyun ^{[1
]}

Kim, Seongcheol ^{[1
]}

Lee, Seong-Wook ^{[1
,2
]}

机构：

[1] Rznomics Inc, R&D Ctr, Seongnam 13486, South Korea

[2] Dankook Univ, Res Inst Adv Omics, Dept Bioconvergence Engn, Yongin 16890, South Korea

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2022年 / 23卷 / 21期

基金：

新加坡国家研究基金会;

关键词：

circular RNA; circularization; RNA; RNA vaccine; RNA therapeutics; ENHANCES TRANSLATION; CYANOGEN-BROMIDE; DNA DUPLEXES; SELF; LIGATION; INTRON; MECHANISMS; DESIGN; LIGASE; STRAND;

D O I：

10.3390/ijms232113247

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

mRNA is gaining success as a new therapeutic agent and vaccine. However, mRNA has limitations in stability. To overcome the shortcomings of mRNA, circular RNA is emerging as a new modality. In this review, several current methods of manufacturing circular RNA in vitro are introduced and their advantages and disadvantages are reviewed. Furthermore, this study discusses which fields and directions of research and development are needed for the increase in the efficacy and productivity of circular RNA as a therapeutic agent and vaccine formulation.

引用

页数：10

共 60 条

[1] Incorporation of pseudouridine into mRNA enhances translation by diminishing PKR activation [J].