Enantioseparations in nonaqueous capillary liquid chromatography and capillary electro-chromatography using cellulose tris(3,5-dimethyl-phenylcarbamate) as chiral stationary phase

被引：0

作者：

Chankvetadze, L

Kartozia, I

Yamamoto, C

Chankvetadze, B

Blaschke, G

Okamoto, Y ^{[1
]}

机构：

[1] Nagoya Univ, Grad Sch Engn, Dept Appl Chem, Nagoya, Aichi, Japan

[2] Univ Munster, Inst Pharmaceut & Med Chem, Munster, Germany

[3] Tbilisi State Univ, Sch Chem, Mol Recognit & Separat Sci Lab, Tbilisi, Georgia

来源：

ELECTROPHORESIS | 2002年 / 23卷 / 03期

关键词：

capillary electrochromatography; cellulose tris(3,5-dimethylphenylcarbamate); chiral selector; enantioseparation;

D O I：

10.1002/1522-2683(200202)23:3<486::AID-ELPS486>3.0.CO;2-L

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The potential of the widely used chiral stationary phase for high-performance liquid chromatography (HPLC) enantioseparations, cellulose tris(3,5-dimethylphenylcarbamate) (CDMPC, sold under the trade name Chiralcel OD) was evaluated under the conditions of nonaqueous capillary electrochromatography (CEC). The effect of the particle size of the silica gel, the loading of CDMPC on the silica gel and nature of the organic solvent, as well as electrolyte salts on the separation characteristics were investigated. This study illustrates the applicability of CDMPC for obtaining highly efficient enantioseparations under the conditions of nonaqueous CEC. Comparative study of enantioseparations in capillary liquid chromatography (CLC) and CEC indicated the significant advantages of CEC such as higher plate number at the similar linear flow rates of the mobile phase as well as better tolerance of higher linear flow rates.

引用

页码：486 / 493

页数：8

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