In-Depth Analysis of the Extracorporeal Proteome Adsorbed to Dialysis Membranes during Hemodialysis

被引:2
|
作者
Daniel-Fischer, Lisa [1 ,2 ]
Sobieszek, Isabel J. [1 ,2 ]
Wagner, Anja [1 ,2 ]
Sacnun, Juan Manuel [1 ,2 ]
Watschinger, Bruno [3 ]
Aufricht, Christoph [1 ]
Kratochwill, Klaus [1 ,2 ]
Herzog, Rebecca [1 ,2 ]
机构
[1] Med Univ Vienna, Comprehens Ctr Pediat, Dept Pediat & Adolescent Med, Div Pediat Nephrol & Gastroenterol, A-1090 Vienna, Austria
[2] Med Univ Vienna, Christian Doppler Lab Mol Stress Res Peritoneal D, A-1090 Vienna, Austria
[3] Med Univ Vienna, Dept Inner Med 3, Div Nephrol & Dialysis, A-1090 Vienna, Austria
基金
奥地利科学基金会;
关键词
hemodialyzer; protein loss; protein adsorption; proteome of hemodialyzer membranes; COMPLEMENT ACTIVATION; ADSORPTION; BIOCOMPATIBILITY; PROTEINS;
D O I
10.3390/membranes12111120
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Used hemodialysis membranes (HD-M) are a valuable reservoir of biological information. Proteins bind to HD-M, but whether this process depends on the type of membrane or patient factors or selectively affects specific protein classes has not been adequately elucidated. State-of-the-art proteomics techniques are capable of identifying and quantifying this therapy-specific subproteome to enable the analysis of disease- or membrane-induced pathophysiologies. We demonstrate the feasibility of the deep proteomic characterization of the extracorporeal proteome adsorbed to HD-M. A shotgun proteomics approach using nano-flow liquid chromatography coupled to mass-spectrometry identified 1648 unique proteins eluted by a chaotropic buffer from the HD-M of eight patients. In total, 995 proteins were present in all eluates; a more stringent approach showed that a core proteome of 310 proteins could be identified independently in all samples. Stability of the dialyzer proteome was demonstrated by a >90% re-identification rate on longitudinal samples of a single patient. The core proteome showed an overrepresentation of pathways of hemostasis and the immune system, and showed differences in membrane materials (polysulfone vs. helixone). This study demonstrates that optimized conditions combined with high-performance proteomics enable the in-depth exploration of the subproteome bound to HD-M, yielding a stable core proteome that can be exploited to study patient-specific factors and improve hemodialysis therapy.
引用
收藏
页数:13
相关论文
共 50 条
  • [41] In-depth urinary and exosome proteome profiling analysis identifies novel biomarkers for diabetic kidney disease
    Shichun Du
    Linhui Zhai
    Shu Ye
    Le Wang
    Muyin Liu
    Minjia Tan
    Science China Life Sciences, 2023, 66 : 2587 - 2603
  • [42] IN-DEPTH ANALYSIS OF THE SYNAPTIC PLASMA MEMBRANE PROTEOME OF SMALL HIPPOCAMPAL SLICES USING AN INTEGRATED APPROACH
    Qiao, Rui
    Li, Shuiming
    Zhou, Mi
    Chen, Penghui
    Liu, Zhao
    Tang, Min
    Zhou, Jian
    NEUROSCIENCE, 2017, 353 : 119 - 132
  • [43] Dissolving capability difference based sequential extraction: A versatile tool for in-depth membrane proteome analysis
    Fang, Fei
    Zhao, Qun
    Li, Xiao
    Liang, Zhen
    Zhang, Lihua
    Zhang, Yukui
    ANALYTICA CHIMICA ACTA, 2016, 945 : 39 - 46
  • [44] In-depth urinary and exosome proteome profiling analysis identifies novel biomarkers for diabetic kidney disease
    Shichun Du
    Linhui Zhai
    Shu Ye
    Le Wang
    Muyin Liu
    Minjia Tan
    Science China(Life Sciences) , 2023, (11) : 2587 - 2603
  • [45] In-depth analysis of blood plasma proteome for discovery of age-related macular degeneration biomarkers
    Woo, Se Joon
    Kim, Hye-Jung
    Suh, Eui Jin
    Park, Ji Hyun
    Ahn, Jeeyun
    Hwang, Duck Jin
    Lee, Ji Eun
    Park, Kyu Hyung
    Lee, Cheolju
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2013, 54 (15)
  • [46] In-depth urinary and exosome proteome profiling analysis identifies novel biomarkers for diabetic kidney disease
    Shichun Du
    Linhui Zhai
    Shu Ye
    Le Wang
    Muyin Liu
    Minjia Tan
    Science China(Life Sciences), 2023, 66 (11) : 2587 - 2603
  • [47] In-depth urinary and exosome proteome profiling analysis identifies novel biomarkers for diabetic kidney disease
    Du, Shichun
    Zhai, Linhui
    Ye, Shu
    Wang, Le
    Liu, Muyin
    Tan, Minjia
    SCIENCE CHINA-LIFE SCIENCES, 2023, 66 (11) : 2587 - 2603
  • [48] Combination of FASP and StageTip-Based Fractionation Allows In-Depth Analysis of the Hippocampal Membrane Proteome
    Wisniewski, Jacek R.
    Zougman, Alexandre
    Mann, Matthias
    JOURNAL OF PROTEOME RESEARCH, 2009, 8 (12) : 5674 - 5678
  • [49] PULMONARY AND CARDIAC ASSISTANCE DURING HEMODIALYSIS - AN EXPERIMENTAL METHOD FOR OXYGENATION OF BLOOD AND SUPPORT OF HEART DURING EXTRACORPOREAL DIALYSIS
    AWAD, JA
    BRASSARD, A
    BINET, J
    CARON, WM
    JOURNAL OF UROLOGY, 1970, 103 (04): : 388 - &
  • [50] IPTH VALUES DURING HEMODIALYSIS - ROLE OF IONIZED CA, DIALYSIS MEMBRANES AND IPTH ASSAYS
    DAMOUR, P
    JOBIN, J
    HAMEL, L
    LECUYER, N
    KIDNEY INTERNATIONAL, 1990, 38 (02) : 308 - 314