Purification and characterization of solvent stable, alkaline protease from Bacillus firmus CAS 7 by microbial conversion of marine wastes and molecular mechanism underlying solvent stability

被引：61
作者：
Annamalai, Neelamegam [1 ]
Rajeswari, Mayavan Veeramuthu [2 ]
Sahu, Sunil Kumar [2 ]
Balasubramanian, Thangavel [2 ]
机构：
[1] Univ Puerto Rico Cayey, Dept Chem, Cayey, PR 00736 USA
[2] Annamalai Univ, Fac Marine Sci, CAS Marine Biol, Parangipettai 608502, TN, India
来源：
PROCESS BIOCHEMISTRY | 2014年 / 49卷 / 06期
关键词：
Bacillus firmus; Marine wastes; Protease; Utilization; MDS; Solvents; HYDRATION; PROTEINASE; BACTERIUM; ENZYMES; SHRIMP; WATER;
D O I：
10.1016/j.procbio.2014.03.007
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A marine bacterium Bacillus firmus CAS 7 produced protease in the medium supplemented with 3:1 shrimp and crab shell powder at 55 C and was purified with the specific activity of 473.4 U/mg. The purified protease was highly stable up to 70 C, pH 11.0 and 30% NaCI. The protease purified was quite stable in the presence of anionic and non-ionic surfactants and organic solvents. The molecular dynamics simulation confirmed that the competition between organic solvent and water for the enzyme surface was comparatively higher in water miscible organic solvent which is responsible for organic solvent stability. The purified protease from B.firmus CAS 7 could be greatly useful to develop industrial processes performed under harsh conditions or with denaturants and organic solvents. The protease production by microbial conversion of marine wastes suggested its potential utilization to generate high value-added products. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码：1012 / 1019
页数：8
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