Forced expression of FLO11 confers pellicle-forming ability and furfural tolerance on Saccharomyces cerevisiae in ethanol production

被引：1

作者：

Nakagawa, Youji ^{[1
]}

Hasebe, Toru ^{[1
]}

Ishiai, Miyuki ^{[1
]}

Yamamura, Hideki ^{[1
]}

Iimura, Yuzuru ^{[1
]}

Hayakawa, Masayuki ^{[1
]}

机构：

[1] Univ Yamanashi, Div Biotechnol, Interdisciplinary Grad Sch Med & Engn, Kofu, Yamanashi 4008511, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2014年 / 78卷 / 04期

关键词：

pellicle formation; FLO11; furfural; bioethanol; Saccharomyces cerevisiae; BIOFILM FORMATION; GENE DISRUPTION; YEAST; STRAIN; RESISTANCE; EVOLUTION; DELETION; WINE;

D O I：

10.1080/09168451.2014.895660

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We constructed a plasmid that expresses FLO11 encoding a cell surface glycoprotein of Saccharomyces cerevisiae under the control of a constitutive promoter. This plasmid conferred pellicle-forming ability on the non-pellicle-forming industrial strain of S. cerevisiae at the air-liquid interface of the glucose-containing liquid medium. The induced pellicle-forming cells exhibited tolerance to furfural, which is a key toxin in lignocellulosic hydrolysates, in ethanol production.

引用

页码：714 / 717

页数：4

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