Forced expression of FLO11 confers pellicle-forming ability and furfural tolerance on Saccharomyces cerevisiae in ethanol production

被引：1

作者：

Nakagawa, Youji ^{[1
]}

Hasebe, Toru ^{[1
]}

Ishiai, Miyuki ^{[1
]}

Yamamura, Hideki ^{[1
]}

Iimura, Yuzuru ^{[1
]}

Hayakawa, Masayuki ^{[1
]}

机构：

[1] Univ Yamanashi, Div Biotechnol, Interdisciplinary Grad Sch Med & Engn, Kofu, Yamanashi 4008511, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2014年 / 78卷 / 04期

关键词：

pellicle formation; FLO11; furfural; bioethanol; Saccharomyces cerevisiae; BIOFILM FORMATION; GENE DISRUPTION; YEAST; STRAIN; RESISTANCE; EVOLUTION; DELETION; WINE;

D O I：

10.1080/09168451.2014.895660

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We constructed a plasmid that expresses FLO11 encoding a cell surface glycoprotein of Saccharomyces cerevisiae under the control of a constitutive promoter. This plasmid conferred pellicle-forming ability on the non-pellicle-forming industrial strain of S. cerevisiae at the air-liquid interface of the glucose-containing liquid medium. The induced pellicle-forming cells exhibited tolerance to furfural, which is a key toxin in lignocellulosic hydrolysates, in ethanol production.

引用

页码：714 / 717

页数：4

共 22 条

[1] Furfural induces reactive oxygen species accumulation and cellular damage in Saccharomyces cerevisiae
Allen, Sandra A.
Clark, William
McCaffery, J. Michael
Cai, Zhen
Lanctot, Alison
Slininger, Patricia J.
Liu, Z. Lewis
Gorsich, Steven W.
[J]. BIOTECHNOLOGY FOR BIOFUELS, 2010, 3
[2] Increased tolerance and conversion of inhibitors in lignocellulosic hydrolysates by Saccharomyces cerevisiae
Almeida, Jodo R. M.
Modig, Tobias
Petersson, Anneli
Hahn-Hagerdal, Barbel
Liden, Gunnar
Gorwa-Grauslund, Marie F.
[J]. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, 2007, 82 (04) : 340 - 349
[3] Brachmann CB, 1998, YEAST, V14, P115
[4] Adaptive evolution by mutations in the FLO11 gene
Fidalgo, Manuel
Barrales, Ramon R.
Ibeas, Jose I.
Jimenez, Juan
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (30) : 11228 - 11233
[5] NEW YEAST-ESCHERICHIA-COLI SHUTTLE VECTORS CONSTRUCTED WITH INVITRO MUTAGENIZED YEAST GENES LACKING 6-BASE PAIR RESTRICTION SITES
GIETZ, RD
SUGINO, A
[J]. GENE, 1988, 74 (02) : 527 - 534
[6] Goldstein AL, 1999, YEAST, V15, P1541, DOI 10.1002/(SICI)1097-0061(199910)15:14<1541::AID-YEA476>3.0.CO
[7] 2-K
[8] Controlled expression of the dominant flocculation genes FLO1, FLO5, and FLO11 in Saccharomyces cerevisiae
Govender, Patrick
Domingo, Jody L.
Bester, Michael C.
Pretorius, Isak S.
Bauer, Florian F.
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2008, 74 (19) : 6041 - 6052
[9] FLO gene-dependent phenotypes in industrial wine yeast strains
Govender, Patrick
Bester, Michael
Bauer, Florian F.
[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2010, 86 (03) : 931 - 945
[10] Identification of furfural as a key toxin in lignocellulosic hydrolysates and evolution of a tolerant yeast strain
Heer, Dominik
Sauer, Uwe
[J]. MICROBIAL BIOTECHNOLOGY, 2008, 1 (06): : 497 - 506

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