Protein denitration/modification by Escherichia coli nitrate reductase and mammalian cytochrome P-450 reductase

被引：7

作者：

Kuo, WN ^{[1
]}

Kocis, JM ^{[1
]}

Webb, J ^{[1
]}

机构：

[1] Bethune Cookman Coll, Div Sci & Math, Daytona Beach, FL 32114 USA

来源：

FRONTIERS IN BIOSCIENCE | 2002年 / 7卷

关键词：

nitric oxide; peroxynitrite; protein nitration/denitration; cytochrome P-450 reductase; nitrate reductase (cytochrome);

D O I：

10.2741/kuo

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The incubation of peroxynitrite (PN)-pretreated histone III-S (NH) with Escherichia coli nitrate reductase (cytochrome, NADPH/GSH-independent) and that of NADPH-treated NH (NHNADPH) with liver cytochrome P-450 reductase (NADPH-dependent) resulted in decreased 3-nitrotyrosine immunoreactivity found in Western blot analysis. Additionally, increased nitrate was noted as an end product of these reactions. These findings imply that varied enzymatic denitration/modification of NO/PN-reacted protein, either with or without a reductant, may be important in regulating related signal transduction cascade(s) and relieving oxidative stress.

引用

页码：A9 / A14

页数：6

共 39 条

[1] Inactivation of tyrosine hydroxylase by nitration following exposure to peroxynitrite and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) [J].

Ara, J ;

Przedborski, S ;

Naini, AB ;

Jackson-Lewis, V ;

Trifiletti, RR ;

Horwitz, J ;

Ischiropoulos, H .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (13) :7659-7663

[2]

Bachmaier K, 1997, CIRCULATION, V96, P585

[3]

BACKES WL, 1993, CYTOCHROME P450

[4] Requirements for heme and thiols for the nonenzymatic modification of nitrotyrosine [J].

Balabanli, B ;

Kamisaki, Y ;

Martin, E ;

Murad, F .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (23) :13136-13141

[5] Protein oxidation in aging, disease, and oxidative stress [J].

Berlett, BS ;

Stadtman, ER .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (33) :20313-20316

[6] Carbon dioxide stimulates peroxynitrite-mediated nitration of tyrosine residues and inhibits oxidation of methionine residues of glutamine synthetase: Both modifications mimic effects of adenylylation [J].

Berlett, BS ;

Levine, RL ;

Stadtman, ER .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (06) :2784-2789

[7]

Browne SE, 1999, BRAIN PATHOL, V9, P147

[8] Differential inhibitory action of nitric oxide and peroxynitrite on mitochondrial electron transport [J].

Cassina, A ;

Radi, R .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1996, 328 (02) :309-316

[9] Oxidative modification of nicotinamide nucleotide transhydrogenase in submitochondrial particles: Effect of endogenous ubiquinol [J].

ForsmarkAndree, P ;

Persson, B ;

Radi, R ;

Dallner, G ;

Ernster, L .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1996, 336 (01) :113-120

[10] Peroxynitrite modification of glutathione reductase: Modeling studies and kinetic evidence suggest the modification of tyrosines at the glutathione disulfide binding site [J].

Francescutti, D ;

Baldwin, J ;

Lee, L ;

Mutus, B .

PROTEIN ENGINEERING, 1996, 9 (02) :189-194

← 1 2 3 4 →