Fe3O4/BSA particles induce osteogenic differentiation of mesenchymal stem cells under static magnetic field

被引：72
作者：
Jiang, Pengfei [1 ]
Zhang, Yixian [1 ]
Zhu, Chaonan [1 ]
Zhang, Wenjing [1 ]
Mao, Zhengwei [1 ]
Gao, Changyou [1 ]
机构：
[1] Zhejiang Univ, Dept Polymer Sci & Engn, MOE Key Lab Macromol Synth & Functionalizat, Hangzhou 310027, Zhejiang, Peoples R China
来源：
ACTA BIOMATERIALIA | 2016年 / 46卷
关键词：
Magnetic particles; Cellular uptake; Magnetic field; Cell differentiation; Mesenchymal stem cell; IRON-OXIDE NANOPARTICLES; DRUG-DELIVERY; BONE-MARROW; IN-VIVO; SUPERPARAMAGNETIC NANOPARTICLES; MATRIX ELASTICITY; CELLULAR UPTAKE; REMOTE-CONTROL; STROMAL CELLS; MRI TRACKING;
D O I：
10.1016/j.actbio.2016.09.020
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Differentiation of stem cells is influenced by many factors, yet uptake of the magnetic particles with or without magnetic field is rarely tackled. In this study, iron oxide nanoparticles-loaded bovine serum albumin (BSA) (Fe3O4/BSA) particles were prepared, which showed a spherical morphology with a diameter below 200 nm, negatively charged surface, and tunable magnetic property. The particles could be internalized into bone marrow mesenchymal stem cells (MSCs), and their release from the cells was significantly retarded under external magnetic field, resulting in almost twice intracellular amount of the particles within 21 d compared to that of the magnetic field free control. Uptake of the Fe3O4/BSA particles enhanced significantly the osteogenic differentiation of MSCs under a static magnetic field, as evidenced by elevated alkaline phosphatase (ALP) activity, calcium deposition, and expressions of collagen type I and osteocalcin at both mRNA and protein levels. Therefore, uptake of the Fe3O4/BSA particles brings significant influence on the differentiation of MSCs under magnetic field, and thereby should be paid great attention for practical applications. Statement of Significance Differentiation of stem cells is influenced by many factors, yet uptake of the magnetic particles with or without magnetic field is rarely tackled. In this study, iron oxide nanoparticles-loaded bovine serum albumin (BSA) (Fe3O4/BSA) particles with a diameter below 200 nm, negatively charged surface, tunable Fe3O4 content and subsequently adjustable magnetic property were prepared. The particles could be internalized into bone marrow mesenchymal stem cells (MSCs), and their release from the cells was significantly retarded under external magnetic field. Uptake of the Fe3O4/BSA particles enhanced significantly the osteogenic differentiation of MSCs under a constant static magnetic field, while the magnetic particles and external magnetic field alone do not influence significantly the osteogenic differentiation potential of MSCs regardless of the uptake amount. The results demonstrate a potential magnetic manipulation method for stem cell differentiation, and also convey the significance of careful evaluation of the safety issue of magnetic particles in real an application situation. (C) 2016 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码：141 / 150
页数：10
相关论文
共 74 条
[31]   Geometric Control of Stem Cell Differentiation Rate on Surfaces [J].
Luo, Wei ;
Jones, Stephanie R. ;
Yousaf, Muhammad N. .
LANGMUIR, 2008, 24 (21) :12129-12133
[32]   Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment [J].
McBeath, R ;
Pirone, DM ;
Nelson, CM ;
Bhadriraju, K ;
Chen, CS .
DEVELOPMENTAL CELL, 2004, 6 (04) :483-495
[33]  
McMurray RJ, 2011, NAT MATER, V10, P637, DOI [10.1038/NMAT3058, 10.1038/nmat3058]
[34]   Gelation Characteristics and Osteogenic Differentiation of Stromal Cells in Inert Hydrolytically Degradable Micellar Polyethylene Glycol Hydrogels [J].
Moeinzadeh, Seyedsina ;
Barati, Danial ;
He, Xuezhong ;
Jabbari, Esmaiel .
BIOMACROMOLECULES, 2012, 13 (07) :2073-2086
[35]   Mesenchymal stem cell fate is regulated by the composition and mechanical properties of collagen-glycosaminoglycan scaffolds [J].
Murphy, Clara M. ;
Matsiko, Amos ;
Haugh, Matthew G. ;
Gleeson, John P. ;
O'Brien, Fergal J. .
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS, 2012, 11 :53-62
[36]   Osteocalcin Secretion as an Early Marker of In Vitro Osteogenic Differentiation of Rat Mesenchymal Stem Cells [J].
Nakamura, Akifumi ;
Dohi, Yoshiko ;
Akahane, Manabu ;
Ohgushi, Hajime ;
Nakajima, Hiroshi ;
Funaoka, Hiroyuki ;
Takakura, Yoshinori .
TISSUE ENGINEERING PART C-METHODS, 2009, 15 (02) :169-180
[37]   BMP2-loaded nanoporous silica nanoparticles promote osteogenic differentiation of human mesenchymal stem cells [J].
Neumann, Anne ;
Christel, Anne ;
Kasper, Cornelia ;
Behrens, Peter .
RSC ADVANCES, 2013, 3 (46) :24222-24230
[38]   Osteogenesis of Mesenchymal Stem Cells by Nanoscale Mechanotransduction [J].
Nikukar, Habib ;
Reid, Stuart ;
Tsimbouri, P. Monica ;
Riehle, Mathis O. ;
Curtis, Adam S. G. ;
Dalby, Matthew J. .
ACS NANO, 2013, 7 (03) :2758-2767
[39]   Stem cell fate dictated solely by altered nanotube dimension [J].
Oh, Seunghan ;
Brammer, Karla S. ;
Li, Y. S. Julie ;
Teng, Dayu ;
Engler, Adam J. ;
Chien, Shu ;
Jin, Sungho .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (07) :2130-2135
[40]   Magnetic Field-Induced T Cell Receptor Clustering by Nanoparticles Enhances T Cell Activation and Stimulates Antitumor Activity [J].
Perica, Karlo ;
Tu, Ang ;
Richter, Anne ;
Bieler, Joan Glick ;
Edidin, Michael ;
Schneck, Jonathan P. .
ACS NANO, 2014, 8 (03) :2252-2260
12345678