Expression and Characterization of a Glutamate Decarboxylase from Lactobacillus brevis 877G Producing γ-Aminobutyric Acid

被引：57

作者：

Seo, Myung-Ji ^{[1
]}

Nam, Young-Do ^{[2
,3
]}

Lee, So-Young ^{[2
]}

Park, So-Lim ^{[2
]}

Sung-Hun, Y. ^{[2
]}

Lim, Seong-Il ^{[2
]}

机构：

[1] Univ Incheon, Div Bioengn, Inchon 406772, South Korea

[2] Korea Food Res Inst, Fermentat & Funct Res Grp, Songnam 463746, South Korea

[3] Univ Sci & Technol, Taejon 305333, South Korea

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2013年 / 77卷 / 04期

关键词：

gamma-aminobutyric acid; characterization; expression; glutamate decarboxylase; Lactobacillus brevis; ENHANCED LEVELS; PURIFICATION; GABA;

D O I：

10.1271/bbb.120785

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The glutamate decarboxylase of gamma-aminobutyric acid-producing Lactobacillus brevis 877G (LbGAD) was expressed in Escherichia coli. The optimal pH and temperature for the purified LbGAD activity were respectively determined to be pH 5.2 and 45 degrees C. CaCl2 was shown to be a potent activator of this LbGAD activity. The kinetic parameters for LbGAD were a K-m value of 3.6 mmol/L and a V-max value of 0.06 mmol/L/min for L-monosodium glutamate.

引用

页码：853 / 856

页数：4

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