Dendritic patterning by Dscam and synaptic partner matching in the Drosophila antennal lobe

被引:126
作者
Zhu, HT
Hummel, T
Clemens, JC
Berdnik, D
Zipursky, SL
Luo, LQ [1 ]
机构
[1] Stanford Univ, Howard Hughes Med Inst, Stanford, CA 94305 USA
[2] Stanford Univ, Dept Biol Sci, Stanford, CA 94305 USA
[3] Univ Calif Los Angeles, Howard Hughes Med Inst, Los Angeles, CA 90095 USA
[4] Univ Calif Los Angeles, David Geffen Sch Med, Dept Biol Chem, Los Angeles, CA 90095 USA
关键词
D O I
10.1038/nn1652
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
In the olfactory system of Drosophila melanogaster, axons of olfactory receptor neurons (ORNs) and dendrites of second-order projection neurons typically target 1 of similar to 50 glomeruli. Dscam, an immunoglobulin superfamily protein, acts in ORNs to regulate axon targeting. Here we show that Dscam acts in projection neurons and local interneurons to control the elaboration of dendritic fields. The removal of Dscam selectively from projection neurons or local interneurons led to clumped dendrites and marked reduction in their dendritic field size. Overexpression of Dscam in projection neurons caused dendrites to be more diffuse during development and shifted their relative position in adulthood. Notably, the positional shift of projection neuron dendrites caused a corresponding shift of its partner ORN axons, thus maintaining the connection specificity. This observation provides evidence for a pre- and postsynaptic matching mechanism independent of precise glomerular positioning.
引用
收藏
页码:349 / 355
页数:7
相关论文
共 35 条
  • [1] A novel family of divergent seven-transmembrane proteins:: Candidate odorant receptors in Drosophila
    Clyne, PJ
    Warr, CG
    Freeman, MR
    Lessing, D
    Kim, JH
    Carlson, JR
    [J]. NEURON, 1999, 22 (02) : 327 - 338
  • [2] COUTO A, 2005, CURR BIOL
  • [3] Integrating the molecular and cellular basis of odor coding in the Drosophila antenna
    Dobritsa, AA
    van der Goes van Naters, W
    Warr, CG
    Steinbrecht, RA
    Carlson, JR
    [J]. NEURON, 2003, 37 (05) : 827 - 841
  • [4] A contextual model for axonal sorting into glomeruli in the mouse olfactory system
    Feinstein, P
    Mombaerts, P
    [J]. CELL, 2004, 117 (06) : 817 - 831
  • [5] Axon guidance of mouse olfactory sensory neurons by odorant receptors and the β2 adrenergic receptor
    Feinstein, P
    Bozza, T
    Rodriguez, I
    Vassalli, A
    Mombaerts, P
    [J]. CELL, 2004, 117 (06) : 833 - 846
  • [6] Genetic and functional subdivision of the Drosophila antennal lobe
    Fishilevich, E
    Vosshall, LB
    [J]. CURRENT BIOLOGY, 2005, 15 (17) : 1548 - 1553
  • [7] Convergent projections of Drosophila olfactory neurons to specific glomeruli in the antennal lobe
    Gao, Q
    Yuan, BB
    Chess, A
    [J]. NATURE NEUROSCIENCE, 2000, 3 (08) : 780 - 785
  • [8] Identification of candidate Drosophila olfactory receptors from genomic DNA sequence
    Gao, Q
    Chess, A
    [J]. GENOMICS, 1999, 60 (01) : 31 - 39
  • [9] Coexpression of two functional odor receptors in one neuron
    Goldman, AL
    van Naters, WV
    Lessing, D
    Warr, CG
    Carlson, JR
    [J]. NEURON, 2005, 45 (05) : 661 - 666
  • [10] Mechanisms of olfactory discrimination: Converging evidence for common principles across phyla
    Hildebrand, JG
    Shepherd, GM
    [J]. ANNUAL REVIEW OF NEUROSCIENCE, 1997, 20 : 595 - 631