Characterisation of anaerobic mixed cultures by partition in an aqueous two-phase system

被引：0
|
作者：
Murto, M [1 ]
Dissing, U [1 ]
Mattiasson, B [1 ]
机构：
[1] Lund Univ, Dept Biotechnol, SE-22100 Lund, Sweden
来源：
BIOTECHNOLOGY LETTERS | 2002年 / 24卷 / 05期
关键词：
anaerobic degradation; aqueous two-phase systems; counter-current distribution; mixed culture; partitioning of cells;
D O I：
10.1023/A:1014577206774
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A method to characterise a dynamic microbial consortium is described. By exploiting differences in surface properties between different cells and between cells of different physiological status, it was possible to develop a partition pattern for a mixed culture under different conditions. The separation method used was partition in aqueous two-phase systems and when using a counter current extraction process one could clearly differentiate the partition profile between resting, active and overloaded biomethanation cultures.
引用
收藏
页码：335 / 341
页数：7
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