Refinement of macaque synteny arrangement with respect to the official rheMac2 macaque sequence assembly

被引:19
作者
Roberto, Roberta [1 ]
Misceo, Doriana [1 ]
D'Addabbo, Pietro [1 ]
Archidiacono, Nicoletta [1 ]
Rocchi, Mariano [1 ]
机构
[1] Univ Bari, Dept Genet & Microbiol, I-70126 Bari, Italy
关键词
macaque genome; macaque sequence; macaque synteny assembly;
D O I
10.1007/s10577-008-1255-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have compared the synteny block organization of the official macaque genome sequence assembly (Jan. 2006; rheMac2) with an independent assembly that used a molecular cytogenetic approach. The mapping of four synteny segments, ranging in size from 4 Mb to 24 Mb, was found to be inconsistent between the two datasets. We specifically investigated these discrepancies by appropriate co-hybridization FISH experiments with validated reference probes located outside the area under study. We found that in the macaque rheMac2 release three synteny segments were wrongly mapped and one segment was incorrectly oriented.
引用
收藏
页码:977 / 985
页数:9
相关论文
共 24 条
[1]   Low rate of genomic repatterning in Xenarthra inferred from chromosome painting data [J].
Dobigny, G ;
Yang, F ;
O'Brien, PCM ;
Volobouev, V ;
Kovács, A ;
Pieczarka, JC ;
Ferguson-Smith, MA ;
Robinson, TJ .
CHROMOSOME RESEARCH, 2005, 13 (07) :651-663
[2]   Evolutionary and biomedical insights from the rhesus macaque genome [J].
Gibbs, Richard A. ;
Rogers, Jeffrey ;
Katze, Michael G. ;
Bumgarner, Roger ;
Weinstock, George M. ;
Mardis, Elaine R. ;
Remington, Karin A. ;
Strausberg, Robert L. ;
Venter, J. Craig ;
Wilson, Richard K. ;
Batzer, Mark A. ;
Bustamante, Carlos D. ;
Eichler, Evan E. ;
Hahn, Matthew W. ;
Hardison, Ross C. ;
Makova, Kateryna D. ;
Miller, Webb ;
Milosavljevic, Aleksandar ;
Palermo, Robert E. ;
Siepel, Adam ;
Sikela, James M. ;
Attaway, Tony ;
Bell, Stephanie ;
Bernard, Kelly E. ;
Buhay, Christian J. ;
Chandrabose, Mimi N. ;
Dao, Marvin ;
Davis, Clay ;
Delehaunty, Kimberly D. ;
Ding, Yan ;
Dinh, Huyen H. ;
Dugan-Rocha, Shannon ;
Fulton, Lucinda A. ;
Gabisi, Ramatu Ayiesha ;
Garner, Toni T. ;
Godfrey, Jennifer ;
Hawes, Alicia C. ;
Hernandez, Judith ;
Hines, Sandra ;
Holder, Michael ;
Hume, Jennifer ;
Jhangiani, Shalini N. ;
Joshi, Vandita ;
Khan, Ziad Mohid ;
Kirkness, Ewen F. ;
Cree, Andrew ;
Fowler, R. Gerald ;
Lee, Sandra ;
Lewis, Lora R. ;
Li, Zhangwan .
SCIENCE, 2007, 316 (5822) :222-234
[3]   Against a whole-genome shotgun [J].
Green, P .
GENOME RESEARCH, 1997, 7 (05) :410-417
[4]   ORIGIN OF HUMAN CHROMOSOME-2 - AN ANCESTRAL TELOMERE TELOMERE FUSION [J].
IJDO, JW ;
BALDINI, A ;
WARD, DC ;
REEDERS, ST ;
WELLS, RA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (20) :9051-9055
[5]   REPuter: the manifold applications of repeat analysis on a genomic scale [J].
Kurtz, S ;
Choudhuri, JV ;
Ohlebusch, E ;
Schleiermacher, C ;
Stoye, J ;
Giegerich, R .
NUCLEIC ACIDS RESEARCH, 2001, 29 (22) :4633-4642
[6]   Initial sequencing and analysis of the human genome [J].
Lander, ES ;
Int Human Genome Sequencing Consortium ;
Linton, LM ;
Birren, B ;
Nusbaum, C ;
Zody, MC ;
Baldwin, J ;
Devon, K ;
Dewar, K ;
Doyle, M ;
FitzHugh, W ;
Funke, R ;
Gage, D ;
Harris, K ;
Heaford, A ;
Howland, J ;
Kann, L ;
Lehoczky, J ;
LeVine, R ;
McEwan, P ;
McKernan, K ;
Meldrim, J ;
Mesirov, JP ;
Miranda, C ;
Morris, W ;
Naylor, J ;
Raymond, C ;
Rosetti, M ;
Santos, R ;
Sheridan, A ;
Sougnez, C ;
Stange-Thomann, N ;
Stojanovic, N ;
Subramanian, A ;
Wyman, D ;
Rogers, J ;
Sulston, J ;
Ainscough, R ;
Beck, S ;
Bentley, D ;
Burton, J ;
Clee, C ;
Carter, N ;
Coulson, A ;
Deadman, R ;
Deloukas, P ;
Dunham, A ;
Dunham, I ;
Durbin, R ;
French, L .
NATURE, 2001, 409 (6822) :860-921
[7]   HIGH-RESOLUTION MAPPING OF HUMAN CHROMOSOME-11 BY INSITU HYBRIDIZATION WITH COSMID CLONES [J].
LICHTER, P ;
TANG, CJC ;
CALL, K ;
HERMANSON, G ;
EVANS, GA ;
HOUSMAN, D ;
WARD, DC .
SCIENCE, 1990, 247 (4938) :64-69
[8]   Neocentromeres: New insights into centromere structure, disease development, and karyotype evolution [J].
Marshall, Owen J. ;
Chueh, Anderly C. ;
Wong, Lee H. ;
Choo, K. H. Andy .
AMERICAN JOURNAL OF HUMAN GENETICS, 2008, 82 (02) :261-282
[9]   Molecular cytogenetic resources for chromosome 4 and comparative analysis of phylogenetic chromosome IV in great apes [J].
Marzella, R ;
Viggiano, L ;
Miolla, V ;
Storlazzi, CT ;
Ricco, A ;
Gentile, E ;
Roberto, R ;
Surace, C ;
Fratello, A ;
Mancini, M ;
Archidiacono, N ;
Rocchi, M .
GENOMICS, 2000, 63 (03) :307-313
[10]   A physical map of the human genome [J].
McPherson, JD ;
Marra, M ;
Hillier, L ;
Waterston, RH ;
Chinwalla, A ;
Wallis, J ;
Sekhon, M ;
Wylie, K ;
Mardis, ER ;
Wilson, RK ;
Fulton, R ;
Kucaba, TA ;
Wagner-McPherson, C ;
Barbazuk, WB ;
Gregory, SG ;
Humphray, SJ ;
French, L ;
Evans, RS ;
Bethel, G ;
Whittaker, A ;
Holden, JL ;
McCann, OT ;
Dunham, A ;
Soderlund, C ;
Scott, CE ;
Bentley, DR ;
Schuler, G ;
Chen, HC ;
Jang, WH ;
Green, ED ;
Idol, JR ;
Maduro, VVB ;
Montgomery, KT ;
Lee, E ;
Miller, A ;
Emerling, S ;
Kucherlapati, R ;
Gibbs, R ;
Scherer, S ;
Gorrell, JH ;
Sodergren, E ;
Clerc-Blankenburg, K ;
Tabor, P ;
Naylor, S ;
Garcia, D ;
de Jong, PJ ;
Catanese, JJ ;
Nowak, N ;
Osoegawa, K ;
Qin, SZ .
NATURE, 2001, 409 (6822) :934-941