1,2-Diacylglycerol hydroperoxides induce the generation and release of superoxide anion from human polymorphonuclear leukocytes

被引:24
作者
Yamamoto, Y
Kambayashi, Y
Ito, T
Watanabe, K
Nakano, M
机构
[1] JAPAN IMMUNORES LABS,DEPT PHOTON & FREE RADICAL RES,TAKASAKI,GUMMA 370,JAPAN
[2] TOKYO METROPOLITAN MATSUZAWA HOSP,DEPT NEUROL,SETAGAYA KU,TOKYO 156,JAPAN
[3] TOKAI UNIV,SCH MED,DEPT PATHOL,ISEHARA,KANAGAWA 25911,JAPAN
关键词
diacylglycerol hydroperoxide; human polymorphonuclear leukocyte; superoxide anion; 2-methyl-6-(p-methoxyphenyl)-3,7-dihydroimidazo[1,2-a]pyrazin-3-one; protein kinase;
D O I
10.1016/S0014-5793(97)00823-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We examined the effect of 1-palmitoyl-2-linoleoylglycerol (PLG), PLG hydroxide (PLG-OH), and PLG hydroperoxide (PLG-OOH) on the release of superoxide anion from human PMNs monitored by the chemiluminescence generated by the superoxide anion-sensitive reagent, 2-methyl-6-(p-methoxyphenyl)-3,7-dihydroimidazo[1,2-a]pyrazin-(MCLA). PLG-OOH at low micromolar concentrations stimulated human PMNs whereas PLG and PEG-OH did not. 1,3-Dilinoleoylglycerol hydroperoxide, 1-palmitoyl-2-linoleoylphosphatidylcholine hydroperoxide, and linoleic acid hydroperoxide were much less efficient in stimulating human PMNs than PLG-OOH. The PKC inhibitors, chelerythrine chloride and staurosporine, inhibited the stimulation of PMNs. Possible pathophysiological role of 1,2-diacylglycerol hydroperoxides is discussed. (C) 1997 Federation of European Biochemical Societies.
引用
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页码:461 / 464
页数:4
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