Evaluation of cytocompatibility and osteoconductivity of Zr-14Nb-5Ta-1Mo alloy with MC3T3-E1 cells

被引：2
作者：
Sato, Hiromitsu [1 ]
Chen, Peng [2 ]
Ashida, Maki [2 ]
Tsutsumi, Yusuke [2 ,3 ]
Harada, Hiroyuki [1 ]
Hanawa, Takao [2 ,4 ]
机构：
[1] Tokyo Med & Dent Univ TMDU, Grad Sch, Div Oral Hlth Sci, Oral & Maxillofacial Surg,Bunkyo Ku, 1-5-45 Yushima, Tokyo 1138510, Japan
[2] Tokyo Med & Dent Univ TMDU, Inst Biomat & Bioengn, Chiyoda Ku, 2-3-10 Kanda Surugadai, Tokyo 1010062, Japan
[3] Natl Inst Mat Sci NIMS, Res Ctr Struct Mat, 1-2-1 Sengen, Tsukuba, Ibaraki 3050047, Japan
[4] Kobe Univ, Ctr Adv Med Engn Res & Dev, Chuo Ku, 1-5-1 Minatojima Minamimachi, Kobe, Hyogo 6500047, Japan
来源：
DENTAL MATERIALS JOURNAL | 2022年 / 41卷 / 03期
基金：
日本学术振兴会;
关键词：
Zirconium alloy; Cytocompatibility; Osteogenic differentiation; Calcification; HIGH-PRESSURE TORSION; MAGNETIC-SUSCEPTIBILITY; ALPHA-2-BETA-1; INTEGRIN; MECHANICAL-PROPERTIES; ALKALINE-PHOSPHATASE; TITANIUM; ZIRCONIUM; OSSEOINTEGRATION; CALCIFICATION; DIFFERENTIATION;
D O I：
10.4012/dmj.2021-169
中图分类号：
R78 [口腔科学];
学科分类号：
1003 ;
摘要：
The cytocompatibility and osteoconductivity of the Zr-14Nb-5Ta-1Mo alloy were investigated using a mouse osteoblastic cell line (MC3T3-E1) to promote the application of this newly developed alloy in dental/medical treatment. The initial cell-attached morphology was visualized by fluorescent staining, and cells cultured on the Zr alloy showed similar cell adhesion behavior to cells cultured on titanium (Ti). In our 5-day proliferation investigation, similar cell numbers were obtained with both Zr alloy and Ti. These results indicate that the cytocompatibility of Zr alloy is similar to that of Ti. In addition, the similar results in the evaluation of alkaline phosphatase (ALP) activity and staining of deposited calcium using alizarin red S with both Zr alloy and Ti indicated that the osteoconductivity of the Zr alloy is similar to that of Ti. Our results prove the good cytocompatibility and osteoconductivity of the Zr-14Nb-5Ta-1Mo alloy, enabling its promotion for use in dental/medical applications.
引用
收藏
页码：421 / 428
页数：8
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