Evaluation of cytocompatibility and osteoconductivity of Zr-14Nb-5Ta-1Mo alloy with MC3T3-E1 cells

被引：2
作者：
Sato, Hiromitsu [1 ]
Chen, Peng [2 ]
Ashida, Maki [2 ]
Tsutsumi, Yusuke [2 ,3 ]
Harada, Hiroyuki [1 ]
Hanawa, Takao [2 ,4 ]
机构：
[1] Tokyo Med & Dent Univ TMDU, Grad Sch, Div Oral Hlth Sci, Oral & Maxillofacial Surg,Bunkyo Ku, 1-5-45 Yushima, Tokyo 1138510, Japan
[2] Tokyo Med & Dent Univ TMDU, Inst Biomat & Bioengn, Chiyoda Ku, 2-3-10 Kanda Surugadai, Tokyo 1010062, Japan
[3] Natl Inst Mat Sci NIMS, Res Ctr Struct Mat, 1-2-1 Sengen, Tsukuba, Ibaraki 3050047, Japan
[4] Kobe Univ, Ctr Adv Med Engn Res & Dev, Chuo Ku, 1-5-1 Minatojima Minamimachi, Kobe, Hyogo 6500047, Japan
来源：
DENTAL MATERIALS JOURNAL | 2022年 / 41卷 / 03期
基金：
日本学术振兴会;
关键词：
Zirconium alloy; Cytocompatibility; Osteogenic differentiation; Calcification; HIGH-PRESSURE TORSION; MAGNETIC-SUSCEPTIBILITY; ALPHA-2-BETA-1; INTEGRIN; MECHANICAL-PROPERTIES; ALKALINE-PHOSPHATASE; TITANIUM; ZIRCONIUM; OSSEOINTEGRATION; CALCIFICATION; DIFFERENTIATION;
D O I：
10.4012/dmj.2021-169
中图分类号：
R78 [口腔科学];
学科分类号：
1003 ;
摘要：
The cytocompatibility and osteoconductivity of the Zr-14Nb-5Ta-1Mo alloy were investigated using a mouse osteoblastic cell line (MC3T3-E1) to promote the application of this newly developed alloy in dental/medical treatment. The initial cell-attached morphology was visualized by fluorescent staining, and cells cultured on the Zr alloy showed similar cell adhesion behavior to cells cultured on titanium (Ti). In our 5-day proliferation investigation, similar cell numbers were obtained with both Zr alloy and Ti. These results indicate that the cytocompatibility of Zr alloy is similar to that of Ti. In addition, the similar results in the evaluation of alkaline phosphatase (ALP) activity and staining of deposited calcium using alizarin red S with both Zr alloy and Ti indicated that the osteoconductivity of the Zr alloy is similar to that of Ti. Our results prove the good cytocompatibility and osteoconductivity of the Zr-14Nb-5Ta-1Mo alloy, enabling its promotion for use in dental/medical applications.
引用
收藏
页码：421 / 428
页数：8
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共 56 条
  • [1] Osteogenic differentiation of bone marrow-derived mesenchymal stem cells on anodized niobium surface
    Antonini, Leonardo Marasca
    Menezes, Tiago Lemos
    dos Santos, Adilar Goncalves, Jr.
    Takimi, Antonio Shigueaki
    Villarinho, Denis Jardim
    dos Santos, Bruno Paiva
    Camassola, Melissa
    Marcuzzo, Jossano Saldanha
    Malfatti, Celia de Fraga
    [J]. JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, 2019, 30 (09)
  • [2] Design of Zirconium Quaternary System Alloys and Their Properties
    Ashida, Maki
    Tsutsumi, Yusuke
    Homma, Kou
    Chen, Peng
    Shimojo, Masayuki
    Hanawa, Takao
    [J]. MATERIALS TRANSACTIONS, 2020, 61 (04) : 776 - 781
  • [3] Effects of Cold Swaging on Mechanical Properties and Magnetic Susceptibility of the Zr-1Mo Alloy
    Ashida, Maki
    Morita, Masahiro
    Tsutsumi, Yusuke
    Nomura, Naoyuki
    Doi, Hisashi
    Chen, Peng
    Hanawa, Takao
    [J]. METALS, 2018, 8 (06)
  • [4] Development of bone-like zirconium oxide nanoceramic modified chitosan based porous nanocomposites for biomedical application
    Bhowmick, Arundhati
    Pramanik, Nilkamal
    Jana, Piyali
    Mitra, Tapas
    Gnanamani, Arumugam
    Das, Manas
    Kundu, Patit Paban
    [J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2017, 95 : 348 - 356
  • [5] Multifunctional zirconium oxide doped chitosan based hybrid nanocomposites as bone tissue engineering materials
    Bhowmick, Arundhati
    Jana, Piyali
    Pramanik, Nilkamal
    Mitra, Tapas
    Banerjee, Sovan Lal
    Gnanamani, Arumugam
    Das, Manas
    Kundu, Patit Paban
    [J]. CARBOHYDRATE POLYMERS, 2016, 151 : 879 - 888
  • [6] Osseointegration of titanium, titanium alloy and zirconia dental implants: current knowledge and open questions
    Bosshardt, Dieter D.
    Chappuis, Vivianne
    Buser, Daniel
    [J]. PERIODONTOLOGY 2000, 2017, 73 (01) : 22 - 40
  • [7] OSSEOINTEGRATION AND ITS EXPERIMENTAL BACKGROUND
    BRANEMARK, PI
    [J]. JOURNAL OF PROSTHETIC DENTISTRY, 1983, 50 (03) : 399 - 410
  • [8] Fatigue Property and Cytocompatibility of a Biomedical Co-Cr-Mo Alloy Subjected to a High Pressure Torsion and a Subsequent Short Time Annealing
    Chen, Peng
    Liu, Huihong
    Niinomi, Mitsuo
    Horita, Zenji
    Fujii, Hidetoshi
    Hanawa, Takao
    [J]. MATERIALS TRANSACTIONS, 2020, 61 (02) : 361 - 367
  • [9] Adhesion and differentiation behaviors of mesenchymal stem cells on titanium with micrometer and nanometer-scale grid patterns produced by femtosecond laser irradiation
    Chen, Peng
    Aso, Toshihiro
    Sasaki, Ryuichiro
    Ashida, Maki
    Tsutsumi, Yusuke
    Doi, Hisashi
    Hanawa, Takao
    [J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2018, 106 (10) : 2735 - 2743
  • [10] Response of preosteoblasts to titanium with periodic micro/nanometer scale grooves produced by femtosecond laser irradiation
    Chen, Peng
    Miyake, Masayoshi
    Tsukamoto, Masahiro
    Tsutsumi, Yusuke
    Hanawa, Takao
    [J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2017, 105 (12) : 3456 - 3464
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